Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding
Abstract To investigate the molecular mechanism of the defense response of “Cabernet Sauvignon” grapes to feeding by Apolygus lucorum, high‐throughput sequencing technology was used to analyze the transcriptome of grape leaves under three different treatments: feeding by A. lucorum, puncture injury,...
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| Format: | Article |
| Language: | English |
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Wiley
2024-05-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.590 |
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| author | Heng Yao Suhong Gao Tianhua Sun Guona Zhou Changkuan Lu Baojia Gao Wenshu Chen Yiming Liang |
| author_facet | Heng Yao Suhong Gao Tianhua Sun Guona Zhou Changkuan Lu Baojia Gao Wenshu Chen Yiming Liang |
| author_sort | Heng Yao |
| collection | DOAJ |
| description | Abstract To investigate the molecular mechanism of the defense response of “Cabernet Sauvignon” grapes to feeding by Apolygus lucorum, high‐throughput sequencing technology was used to analyze the transcriptome of grape leaves under three different treatments: feeding by A. lucorum, puncture injury, and an untreated control. The research findings indicated that the differentially expressed genes were primarily enriched in three aspects: cellular composition, molecular function, and biological process. These genes were found to be involved in 42 metabolic pathways, particularly in plant hormone signaling metabolism, plant‐pathogen interaction, MAPK signaling pathway, and other metabolic pathways associated with plant‐induced insect resistance. Feeding by A. lucorum stimulated and upregulated a significant number of genes related to jasmonic acid and calcium ion pathways, suggesting their crucial role in the defense molecular mechanism of “Cabernet Sauvignon” grapes. The consistency between the gene expression and transcriptome sequencing results further supports these findings. This study provides a reference for the further exploration of the defense response in “Cabernet Sauvignon” grapes by elucidating the expression of relevant genes during feeding by A. lucorum. |
| format | Article |
| id | doaj-art-88cab0c9324e48d5bcf76ea44ea9e941 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-88cab0c9324e48d5bcf76ea44ea9e9412025-02-04T08:30:59ZengWileyPlant Direct2475-44552024-05-0185n/an/a10.1002/pld3.590Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feedingHeng Yao0Suhong Gao1Tianhua Sun2Guona Zhou3Changkuan Lu4Baojia Gao5Wenshu Chen6Yiming Liang7College of Agronomy and Biotechnology Hebei Normal University of Science and Technology Changli Hebei ChinaCollege of Agronomy and Biotechnology Hebei Normal University of Science and Technology Changli Hebei ChinaCollege of Forestry Hebei Agricultural University Baoding Hebei ChinaCollege of Forestry Hebei Agricultural University Baoding Hebei ChinaCollege of Agronomy and Biotechnology Hebei Normal University of Science and Technology Changli Hebei ChinaCollege of Forestry Hebei Agricultural University Baoding Hebei ChinaCollege of Agronomy and Biotechnology Hebei Normal University of Science and Technology Changli Hebei ChinaCollege of Agronomy and Biotechnology Hebei Normal University of Science and Technology Changli Hebei ChinaAbstract To investigate the molecular mechanism of the defense response of “Cabernet Sauvignon” grapes to feeding by Apolygus lucorum, high‐throughput sequencing technology was used to analyze the transcriptome of grape leaves under three different treatments: feeding by A. lucorum, puncture injury, and an untreated control. The research findings indicated that the differentially expressed genes were primarily enriched in three aspects: cellular composition, molecular function, and biological process. These genes were found to be involved in 42 metabolic pathways, particularly in plant hormone signaling metabolism, plant‐pathogen interaction, MAPK signaling pathway, and other metabolic pathways associated with plant‐induced insect resistance. Feeding by A. lucorum stimulated and upregulated a significant number of genes related to jasmonic acid and calcium ion pathways, suggesting their crucial role in the defense molecular mechanism of “Cabernet Sauvignon” grapes. The consistency between the gene expression and transcriptome sequencing results further supports these findings. This study provides a reference for the further exploration of the defense response in “Cabernet Sauvignon” grapes by elucidating the expression of relevant genes during feeding by A. lucorum.https://doi.org/10.1002/pld3.590Apolygus lucorumCabernet Sauvignondefense responsedifferentially expressed genemetabolic pathwaytranscriptome sequencing |
| spellingShingle | Heng Yao Suhong Gao Tianhua Sun Guona Zhou Changkuan Lu Baojia Gao Wenshu Chen Yiming Liang Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding Plant Direct Apolygus lucorum Cabernet Sauvignon defense response differentially expressed gene metabolic pathway transcriptome sequencing |
| title | Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding |
| title_full | Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding |
| title_fullStr | Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding |
| title_full_unstemmed | Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding |
| title_short | Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding |
| title_sort | transcriptomic analysis of the defense response in cabernet sauvignon grape leaf induced by apolygus lucorum feeding |
| topic | Apolygus lucorum Cabernet Sauvignon defense response differentially expressed gene metabolic pathway transcriptome sequencing |
| url | https://doi.org/10.1002/pld3.590 |
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