Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro
This study represents the first evidence of the bacterial blight caused by Xanthomonas arboricola pv. juglandis (Xaj) on walnut trees in Podgorica (Montenegro). Disease symptoms appeared on leaves in the form of dark, angular leaf spots surrounded by yellow-green haloes and lesions spread across the...
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Czech Academy of Agricultural Sciences
2025-07-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202503-0004_evidence-and-characterisation-of-xanthomonas-arboricola-pv-juglandis-causing-bacterial-blight-of-walnut-in-mon.php |
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| author | Jelena Latinović Nedeljko Latinović Bogoljub Kandić Aleksandra Jelušić Tatjana Popović Milovanović |
| author_facet | Jelena Latinović Nedeljko Latinović Bogoljub Kandić Aleksandra Jelušić Tatjana Popović Milovanović |
| author_sort | Jelena Latinović |
| collection | DOAJ |
| description | This study represents the first evidence of the bacterial blight caused by Xanthomonas arboricola pv. juglandis (Xaj) on walnut trees in Podgorica (Montenegro). Disease symptoms appeared on leaves in the form of dark, angular leaf spots surrounded by yellow-green haloes and lesions spread across the whole leaf. Isolated bacteria were preliminarily identified using PCR with pathovar-specific primer pair XajF/XajR and further characterised based on multi-locus sequence analysis with nine housekeeping genes (fusA, gapA, gltA, gyrB1, lepA, rpoD, dnaK, fyuA, and gyrB2). Montenegrin walnut isolates were homogeneous among themselves and the most closely related to different X. arboricola strains originating from Juglans regia isolated elsewhere. The pathogenicity of isolates was confirmed on walnut leaves, fruits, and branches. All inoculations resulted in the formation of necrotic lesions that initially developed at the site of bacteria entry, with later developing chlorotic areas on leaves along the leaf veins. This finding of Xaj causing leaf blight symptoms on walnuts in Montenegro highlights its expanding distribution across Europe and indicates a potential threat to walnut plantations in Montenegro. |
| format | Article |
| id | doaj-art-88c5a61bb0c442b2afd39e345dc59e62 |
| institution | DOAJ |
| issn | 1212-2580 1805-9341 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj-art-88c5a61bb0c442b2afd39e345dc59e622025-08-20T02:50:53ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412025-07-0161325526110.17221/196/2024-PPSpps-202503-0004Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in MontenegroJelena Latinović0Nedeljko Latinović1Bogoljub Kandić2Aleksandra Jelušić3Tatjana Popović Milovanović4https://orcid.org/0000-0003-0125-5905University of Montenegro, Biotechnical Faculty, Podgorica, MontenegroUniversity of Montenegro, Biotechnical Faculty, Podgorica, MontenegroUniversity of Montenegro, Biotechnical Faculty, Podgorica, MontenegroUniversity of Belgrade, Institute for Multidisciplinary Research, Belgrade, SerbiaInstitute for Plant Protection and Environment, Belgrade, SerbiaThis study represents the first evidence of the bacterial blight caused by Xanthomonas arboricola pv. juglandis (Xaj) on walnut trees in Podgorica (Montenegro). Disease symptoms appeared on leaves in the form of dark, angular leaf spots surrounded by yellow-green haloes and lesions spread across the whole leaf. Isolated bacteria were preliminarily identified using PCR with pathovar-specific primer pair XajF/XajR and further characterised based on multi-locus sequence analysis with nine housekeeping genes (fusA, gapA, gltA, gyrB1, lepA, rpoD, dnaK, fyuA, and gyrB2). Montenegrin walnut isolates were homogeneous among themselves and the most closely related to different X. arboricola strains originating from Juglans regia isolated elsewhere. The pathogenicity of isolates was confirmed on walnut leaves, fruits, and branches. All inoculations resulted in the formation of necrotic lesions that initially developed at the site of bacteria entry, with later developing chlorotic areas on leaves along the leaf veins. This finding of Xaj causing leaf blight symptoms on walnuts in Montenegro highlights its expanding distribution across Europe and indicates a potential threat to walnut plantations in Montenegro.https://pps.agriculturejournals.cz/artkey/pps-202503-0004_evidence-and-characterisation-of-xanthomonas-arboricola-pv-juglandis-causing-bacterial-blight-of-walnut-in-mon.phpjuglans regiadetectionidentificationpathogenicitymulti-locus sequence analysis |
| spellingShingle | Jelena Latinović Nedeljko Latinović Bogoljub Kandić Aleksandra Jelušić Tatjana Popović Milovanović Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro Plant Protection Science juglans regia detection identification pathogenicity multi-locus sequence analysis |
| title | Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro |
| title_full | Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro |
| title_fullStr | Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro |
| title_full_unstemmed | Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro |
| title_short | Evidence and characterisation of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnut in Montenegro |
| title_sort | evidence and characterisation of xanthomonas arboricola pv juglandis causing bacterial blight of walnut in montenegro |
| topic | juglans regia detection identification pathogenicity multi-locus sequence analysis |
| url | https://pps.agriculturejournals.cz/artkey/pps-202503-0004_evidence-and-characterisation-of-xanthomonas-arboricola-pv-juglandis-causing-bacterial-blight-of-walnut-in-mon.php |
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