Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants
Huanglongbing disease (HLB) is the most severe citrus disease destroying Citrus reticulata L. ‘Kinnow’, the most commonly grown mandarin in Pakistan. It is caused by Candidatus Liberibacter bacterial species and it spreads through the sucking Asian citrus psyllid insect. The current study was design...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1476497/full |
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| author | Muhammad Ikram Muhammad Ikram Naveed Iqbal Raja Azza H. Mohamed Zia-Ur-Rehman Mashwani Ahmad A. Omar Ahmad A. Omar Hassan Gharibi Roman A. Zubarev Roman A. Zubarev |
| author_facet | Muhammad Ikram Muhammad Ikram Naveed Iqbal Raja Azza H. Mohamed Zia-Ur-Rehman Mashwani Ahmad A. Omar Ahmad A. Omar Hassan Gharibi Roman A. Zubarev Roman A. Zubarev |
| author_sort | Muhammad Ikram |
| collection | DOAJ |
| description | Huanglongbing disease (HLB) is the most severe citrus disease destroying Citrus reticulata L. ‘Kinnow’, the most commonly grown mandarin in Pakistan. It is caused by Candidatus Liberibacter bacterial species and it spreads through the sucking Asian citrus psyllid insect. The current study was designed to investigate the potential impact of plant extract mediated selenium nanoparticles (SeNPs) on antioxidant defense system, fruit quality and protein regulation in the fruits of HLB-infected ‘Kinnow’ mandarin plants. Garlic cloves extract was used as reducing and capping agent for the synthesis of SeNPs. Various concentrations of SeNPs (25, 50, 75, and 100 mg L−1) were exogeneously applied to HLB-positive citrus plants. SeNPs at the concentration of 75 mg L-1 affected positively fruit physio-biochemical parameters, e.g., peel thickness, peel weight, fruit weight, fruit diameter, total soluble solids, juice volume, ascorbic acid content and reduced total acidity. Furthermore, SeNPs also enhanced the amounts of total protein and total sugar as well as elevated antioxidant enzymes, e.g., superoxide dismutase, peroxidases, and catalases. Non-enzymatic antioxidant content, e.g., total phenolic and total flavonoids, was also elevated. Proteomics analysis revealed that exposure to SeNPs at the concentration of 75 mg·L–1 significantly altered in HLB infected mandarin fruting plants the expression of proteins associated with transcription, protection, cell wall biogenesis, cell wall organization, reproduction, stamen formation, embryo development, inflorescence development, as well as translation and response to oxidative stress. Our results revealed that foliar application of SeNPs influences the protein contents positively, therefore ameliorating fruit physio-biochemical quality by boosting antioxidant defense systems of HLB-infected ‘Kinnow’ mandarin plants. |
| format | Article |
| id | doaj-art-88bc2f0427bc40f4a05fea56e5011629 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-88bc2f0427bc40f4a05fea56e50116292025-08-20T02:12:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14764971476497Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plantsMuhammad Ikram0Muhammad Ikram1Naveed Iqbal Raja2Azza H. Mohamed3Zia-Ur-Rehman Mashwani4Ahmad A. Omar5Ahmad A. Omar6Hassan Gharibi7Roman A. Zubarev8Roman A. Zubarev9Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Punjab, PakistanDepartment of Biomedical, Surgical and Dental Sciences, Milan State University, Milan, ItalyDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Punjab, PakistanDepartment of Agricultural Chemistry, College of Agriculture, Mansoura University, Mansoura, EgyptDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Punjab, PakistanBiochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, EgyptCitrus Research and Education Center, University of Florida, IFAS, Lake Alfred, FL, United StatesDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SwedenDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SwedenDepartment of Pharmaceutical and Toxicological Chemistry, Medical Institute, RUDN University, Moscow, RussiaHuanglongbing disease (HLB) is the most severe citrus disease destroying Citrus reticulata L. ‘Kinnow’, the most commonly grown mandarin in Pakistan. It is caused by Candidatus Liberibacter bacterial species and it spreads through the sucking Asian citrus psyllid insect. The current study was designed to investigate the potential impact of plant extract mediated selenium nanoparticles (SeNPs) on antioxidant defense system, fruit quality and protein regulation in the fruits of HLB-infected ‘Kinnow’ mandarin plants. Garlic cloves extract was used as reducing and capping agent for the synthesis of SeNPs. Various concentrations of SeNPs (25, 50, 75, and 100 mg L−1) were exogeneously applied to HLB-positive citrus plants. SeNPs at the concentration of 75 mg L-1 affected positively fruit physio-biochemical parameters, e.g., peel thickness, peel weight, fruit weight, fruit diameter, total soluble solids, juice volume, ascorbic acid content and reduced total acidity. Furthermore, SeNPs also enhanced the amounts of total protein and total sugar as well as elevated antioxidant enzymes, e.g., superoxide dismutase, peroxidases, and catalases. Non-enzymatic antioxidant content, e.g., total phenolic and total flavonoids, was also elevated. Proteomics analysis revealed that exposure to SeNPs at the concentration of 75 mg·L–1 significantly altered in HLB infected mandarin fruting plants the expression of proteins associated with transcription, protection, cell wall biogenesis, cell wall organization, reproduction, stamen formation, embryo development, inflorescence development, as well as translation and response to oxidative stress. Our results revealed that foliar application of SeNPs influences the protein contents positively, therefore ameliorating fruit physio-biochemical quality by boosting antioxidant defense systems of HLB-infected ‘Kinnow’ mandarin plants.https://www.frontiersin.org/articles/10.3389/fpls.2024.1476497/fullCitrus reticulata L. Kinnowmandarinnanobiotechnologyhuanglongbingselenium nanoparticles |
| spellingShingle | Muhammad Ikram Muhammad Ikram Naveed Iqbal Raja Azza H. Mohamed Zia-Ur-Rehman Mashwani Ahmad A. Omar Ahmad A. Omar Hassan Gharibi Roman A. Zubarev Roman A. Zubarev Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants Frontiers in Plant Science Citrus reticulata L. Kinnow mandarin nanobiotechnology huanglongbing selenium nanoparticles |
| title | Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants |
| title_full | Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants |
| title_fullStr | Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants |
| title_full_unstemmed | Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants |
| title_short | Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected ‘Kinnow’ mandarin plants |
| title_sort | differential impact of plant based selenium nanoparticles on physio biochemical properties antioxidant defense system and protein regulation in fruits of huanglongbing infected kinnow mandarin plants |
| topic | Citrus reticulata L. Kinnow mandarin nanobiotechnology huanglongbing selenium nanoparticles |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1476497/full |
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