Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers
Purpose. The study aimed to evaluate the effect of all-trans retinoic acid-loaded nanostructured lipid carriers (ATRA-NLCs) on the zymosan-induced expression of the cytokines IL-4, IL-10, and IFN-γ and the matrix metalloproteinases/tissue inhibitor of metalloproteinases (MMPs/TIMPs) and TLR2 in rabb...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/4952340 |
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| _version_ | 1850211702875881472 |
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| author | Hongyan Zhou Wensong Zhang Xunyi Gao Hongguang Zhang Ning Kong |
| author_facet | Hongyan Zhou Wensong Zhang Xunyi Gao Hongguang Zhang Ning Kong |
| author_sort | Hongyan Zhou |
| collection | DOAJ |
| description | Purpose. The study aimed to evaluate the effect of all-trans retinoic acid-loaded nanostructured lipid carriers (ATRA-NLCs) on the zymosan-induced expression of the cytokines IL-4, IL-10, and IFN-γ and the matrix metalloproteinases/tissue inhibitor of metalloproteinases (MMPs/TIMPs) and TLR2 in rabbit corneal fibroblasts (RCFs). Methods. ATRA-NLCs were prepared by emulsification. RCFs were isolated and harvested after four to seven passages in monolayer culture. Cytokine release (IL-4, IL-10, and IFN-γ) induced by zymosan was analyzed by cytokine release assay, reverse transcription, and real-time polymerase chain reaction (RT-PCR) analysis detection. MMP-1, MMP-3, and MMP-13, TIMP-1 and TIMP-2, and TLR2 expression were analyzed by immunoblotting. Results. ATRA-NLCs were resistant to light and physically stable, and the average size of the ATRA-NLCs was 200 nm. ATRA-NLCs increased the zymosan-induced release of IL-4 and IL-10 and decreased the release of IFN-γ by RCFs. ATRA-NLCs decreased the levels of TLR2 and MMPs/TIMPs above. Conclusions. ATRA may be a potent anti-inflammatory agent for the therapy of fungal keratitis (FK). |
| format | Article |
| id | doaj-art-88bbd053f82145e49d80a5335a9022aa |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-88bbd053f82145e49d80a5335a9022aa2025-08-20T02:09:30ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/49523404952340Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid CarriersHongyan Zhou0Wensong Zhang1Xunyi Gao2Hongguang Zhang3Ning Kong4Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, ChinaDepartment of Ophthalmology, The Second Hospital of Jilin University, Changchun 130000, ChinaDepartment of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, ChinaDepartment of Chemical Engineering and Application, College of Chemistry, Jilin University, Changchun 130000, ChinaDepartment of Pharmacy, College of Pharmacy, Jilin University, Changchun 130041, ChinaPurpose. The study aimed to evaluate the effect of all-trans retinoic acid-loaded nanostructured lipid carriers (ATRA-NLCs) on the zymosan-induced expression of the cytokines IL-4, IL-10, and IFN-γ and the matrix metalloproteinases/tissue inhibitor of metalloproteinases (MMPs/TIMPs) and TLR2 in rabbit corneal fibroblasts (RCFs). Methods. ATRA-NLCs were prepared by emulsification. RCFs were isolated and harvested after four to seven passages in monolayer culture. Cytokine release (IL-4, IL-10, and IFN-γ) induced by zymosan was analyzed by cytokine release assay, reverse transcription, and real-time polymerase chain reaction (RT-PCR) analysis detection. MMP-1, MMP-3, and MMP-13, TIMP-1 and TIMP-2, and TLR2 expression were analyzed by immunoblotting. Results. ATRA-NLCs were resistant to light and physically stable, and the average size of the ATRA-NLCs was 200 nm. ATRA-NLCs increased the zymosan-induced release of IL-4 and IL-10 and decreased the release of IFN-γ by RCFs. ATRA-NLCs decreased the levels of TLR2 and MMPs/TIMPs above. Conclusions. ATRA may be a potent anti-inflammatory agent for the therapy of fungal keratitis (FK).http://dx.doi.org/10.1155/2016/4952340 |
| spellingShingle | Hongyan Zhou Wensong Zhang Xunyi Gao Hongguang Zhang Ning Kong Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers Journal of Ophthalmology |
| title | Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers |
| title_full | Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers |
| title_fullStr | Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers |
| title_full_unstemmed | Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers |
| title_short | Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers |
| title_sort | inhibition of zymosan induced inflammatory factors expression by atra nanostructured lipid carriers |
| url | http://dx.doi.org/10.1155/2016/4952340 |
| work_keys_str_mv | AT hongyanzhou inhibitionofzymosaninducedinflammatoryfactorsexpressionbyatrananostructuredlipidcarriers AT wensongzhang inhibitionofzymosaninducedinflammatoryfactorsexpressionbyatrananostructuredlipidcarriers AT xunyigao inhibitionofzymosaninducedinflammatoryfactorsexpressionbyatrananostructuredlipidcarriers AT hongguangzhang inhibitionofzymosaninducedinflammatoryfactorsexpressionbyatrananostructuredlipidcarriers AT ningkong inhibitionofzymosaninducedinflammatoryfactorsexpressionbyatrananostructuredlipidcarriers |