Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba
Introduction: Urinary tract infection is a major cause of morbidity and mortality worldwide. Uropathogenic Escherichia coli bacteria are the most common cause of urinary tract infections. Drug resistant Escherichia coli is results in high levels of treatment failure and can be a significant threat...
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The Journal of Infection in Developing Countries
2018-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9916 |
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| author | Belayneh Regasa Dadi Tamrat Abebe Lixin Zhang Adane Mihret Workeabeba Abebe Wondwossen Amogne |
| author_facet | Belayneh Regasa Dadi Tamrat Abebe Lixin Zhang Adane Mihret Workeabeba Abebe Wondwossen Amogne |
| author_sort | Belayneh Regasa Dadi |
| collection | DOAJ |
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Introduction: Urinary tract infection is a major cause of morbidity and mortality worldwide. Uropathogenic Escherichia coli bacteria are the most common cause of urinary tract infections. Drug resistant Escherichia coli is results in high levels of treatment failure and can be a significant threat to survival of patients.
Methodology: Escherichia coli bacteria were isolated using culture and conventional biochemical tests. Antimicrobial susceptibility testing and plasmid profile were performed using the Kirby Bauer disc diffusion method and plasmid analysis. Data was processed with SPSS version 16.0 and Epi-info version 3.4.1 software.
Results: The highest proportion of Escherichia coli isolates was resistant to (86.5%) to ampicillin, followed by ceftazidime (84%), ceftriaxone (80.5%), tetracycline (80%), trimethoprim-sulfamethoxazole (68.5%) and cefotaxime (66%). Escherichia coli isolates were most susceptible to meropenem (100%), imipenem (100%), amikacin (97.5%), nitrofurantoin (95%), ciprofloxacin (85.5%), norfloxacin (85%), chloramphenicol (83.5%), gentamycin (80%) and nalidixic acid (79%). Multidrug resistance (MDR) was observed in most (96.5%) E. coli isolates. Plasmid analysis revealed the presence of plasmid(s) in 165 (82.5%) of the E. coli isolates many of which had a plasmid size of 23 kb.
Conclusions: The overall incidence of antibiotic resistance (including MDR) among E. coli in this study was high to commonly used antibiotics, but no drug resistance to meropenem and imipenem was observed. Periodic monitoring of the drug resistance pattern is essential for better management of urinary tract infections, which has direct impact on the outcome of the patient.
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| format | Article |
| id | doaj-art-88acaed6203a49ac98cf0e12dcb53d8e |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-88acaed6203a49ac98cf0e12dcb53d8e2025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-08-01120810.3855/jidc.9916Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis AbebaBelayneh Regasa Dadi0Tamrat Abebe1Lixin Zhang2Adane Mihret3Workeabeba Abebe4Wondwossen Amogne5Arba Minch University, Arba Minch, EthiopiaAddis Ababa University, Addis Abeba, EthiopiaMichigan State University, East Lansing, MI, United StatesAddis Ababa University, Addis Abeba, EthiopiaAddis Ababa University, Addis Abeba, EthiopiaAddis Ababa University, Addis Abeba, Ethiopia Introduction: Urinary tract infection is a major cause of morbidity and mortality worldwide. Uropathogenic Escherichia coli bacteria are the most common cause of urinary tract infections. Drug resistant Escherichia coli is results in high levels of treatment failure and can be a significant threat to survival of patients. Methodology: Escherichia coli bacteria were isolated using culture and conventional biochemical tests. Antimicrobial susceptibility testing and plasmid profile were performed using the Kirby Bauer disc diffusion method and plasmid analysis. Data was processed with SPSS version 16.0 and Epi-info version 3.4.1 software. Results: The highest proportion of Escherichia coli isolates was resistant to (86.5%) to ampicillin, followed by ceftazidime (84%), ceftriaxone (80.5%), tetracycline (80%), trimethoprim-sulfamethoxazole (68.5%) and cefotaxime (66%). Escherichia coli isolates were most susceptible to meropenem (100%), imipenem (100%), amikacin (97.5%), nitrofurantoin (95%), ciprofloxacin (85.5%), norfloxacin (85%), chloramphenicol (83.5%), gentamycin (80%) and nalidixic acid (79%). Multidrug resistance (MDR) was observed in most (96.5%) E. coli isolates. Plasmid analysis revealed the presence of plasmid(s) in 165 (82.5%) of the E. coli isolates many of which had a plasmid size of 23 kb. Conclusions: The overall incidence of antibiotic resistance (including MDR) among E. coli in this study was high to commonly used antibiotics, but no drug resistance to meropenem and imipenem was observed. Periodic monitoring of the drug resistance pattern is essential for better management of urinary tract infections, which has direct impact on the outcome of the patient. https://jidc.org/index.php/journal/article/view/9916uropathogenic Escherichia coliurinary tract infectionsUTIdrug resistanceplasmid profile |
| spellingShingle | Belayneh Regasa Dadi Tamrat Abebe Lixin Zhang Adane Mihret Workeabeba Abebe Wondwossen Amogne Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba Journal of Infection in Developing Countries uropathogenic Escherichia coli urinary tract infections UTI drug resistance plasmid profile |
| title | Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba |
| title_full | Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba |
| title_fullStr | Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba |
| title_full_unstemmed | Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba |
| title_short | Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba |
| title_sort | drug resistance and plasmid profile of uropathogenic escherichia coli among urinary tract infection patients in addis abeba |
| topic | uropathogenic Escherichia coli urinary tract infections UTI drug resistance plasmid profile |
| url | https://jidc.org/index.php/journal/article/view/9916 |
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