Extraction and purification of low content flavonoids from bayberry (Myrica Rubra Sieb.) kernel by the combination of ultrasonic and cellulase techniques
A novel method, the combination of ultrasonic and cellulase techniques, was developed to isolate and purify the flavonoids from Myrica ruba kernel by using the chromatography with macroporous resin. Results showed that the optimum conditions were as follows: the ratio of solid and liquid 1∶10, the a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.02.014 |
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| Summary: | A novel method, the combination of ultrasonic and cellulase techniques, was developed to isolate and purify the flavonoids from Myrica ruba kernel by using the chromatography with macroporous resin. Results showed that the optimum conditions were as follows: the ratio of solid and liquid 1∶10, the amount of cellulase 1.5%, enzymatic hydrolysis temperature 40 ℃, pH of enzyme solution 6.0, enzymatic hydrolysis time 2 h, the concentration of alcohol 50%, and the treatment time for the ultrasonic 4 h. The content of flavonoids in the extractions increased up to 66.10% after purification with D101 macro-porous resin by using 60% ethanol as eluant. The results illustrate that the applied experimental technique is suitable to extract and purify the low content flavonoids in bayberry kernels. |
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| ISSN: | 1008-9209 2097-5155 |