Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro

ABSTRACT Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact...

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Main Authors: Matthew Bolino, Hatice Duman, İzzet Avcı, Hacı Mehmet Kayili, Juli Petereit, Chandler Zundel, Bekir Salih, Sercan Karav, Steven A. Frese
Format: Article
Language:English
Published: American Society for Microbiology 2025-08-01
Series:Microbiology Spectrum
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Online Access:https://journals.asm.org/doi/10.1128/spectrum.00200-25
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author Matthew Bolino
Hatice Duman
İzzet Avcı
Hacı Mehmet Kayili
Juli Petereit
Chandler Zundel
Bekir Salih
Sercan Karav
Steven A. Frese
author_facet Matthew Bolino
Hatice Duman
İzzet Avcı
Hacı Mehmet Kayili
Juli Petereit
Chandler Zundel
Bekir Salih
Sercan Karav
Steven A. Frese
author_sort Matthew Bolino
collection DOAJ
description ABSTRACT Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact with the gut microbiome has highlighted the importance of understanding the nuances underpinning diet-microbiome interactions. Novel food proteins with distinct post-translational modifications resulting from their respective production systems have not been characterized, nor how they may differ from their traditionally produced counterparts. Here, we have characterized the protein composition and N-glycome of a yeast-synthesized and commercially available whey protein ingredient and compared this novel ingredient to whey protein isolate powder derived from bovine milk. Despite strong similarities in protein composition, we found that the N-glycome significantly differs between the two protein sources, reflecting the biosynthetic machinery of the production systems. Furthermore, the diversity of proteins found in yeast-synthesized whey protein were lower relative to bovine whey protein, despite both being predominantly β-lactoglobulin. Finally, to understand whether these differences in N-glycome profiles may affect the human gut microbiome, we compared these proteins in an in vitro fecal fermentation model. The two whey protein sources generated significant differences among three representative gut microbiomes in vitro, most likely due to differences in N-glycan composition and degradation by these representative microbial communities. This work highlights the need to understand how differences in novel biotechnological systems affect the bioactivity of synthesized proteins and how these differences impact the human gut microbiome.IMPORTANCERecent advances in food technology have led to the production of animal-free products from yeast that are traditionally derived from animals, such as milk proteins. These new processes raise important questions about the use of synthetic proteins as a replacement for traditionally sourced protein, especially in the context of the gut microbiome. Importantly, yeast produce N-glycans comprised primarily of mannose, while animals synthesize structurally and compositionally complex N-glycan structures. Given these differences, we characterized a new, yeast-derived whey protein ingredient and compared it to bovine whey protein. We found that yeast-derived whey protein differs in its impact on human gut microbiomes because of differences in N-glycan structures, despite similarity in protein composition. These findings raise important questions as to whether these differences in synthetic proteins lead to significant changes to the gut microbiome in vivo, and whether this may impact the utility of these novel ingredients.
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spelling doaj-art-889c1093bb4a4a34989a0df577de84fe2025-08-20T04:00:44ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-08-0113810.1128/spectrum.00200-25Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitroMatthew Bolino0Hatice Duman1İzzet Avcı2Hacı Mehmet Kayili3Juli Petereit4Chandler Zundel5Bekir Salih6Sercan Karav7Steven A. Frese8Department of Nutrition, University of Nevada, Reno, Reno, Nevada, USADepartment of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale, TurkeyDepartment of Chemistry, Faculty of Science, Hacettepe University, Ankara, TurkeyDepartment of Biomedical Engineering, Faculty of Engineering, Karabuk University, Karabük, TurkeyNevada Bioinformatics Center, University of Nevada, Reno, Reno, Nevada, USADepartment of Nutrition, University of Nevada, Reno, Reno, Nevada, USADepartment of Chemistry, Faculty of Science, Hacettepe University, Ankara, TurkeyDepartment of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale, TurkeyDepartment of Nutrition, University of Nevada, Reno, Reno, Nevada, USAABSTRACT Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact with the gut microbiome has highlighted the importance of understanding the nuances underpinning diet-microbiome interactions. Novel food proteins with distinct post-translational modifications resulting from their respective production systems have not been characterized, nor how they may differ from their traditionally produced counterparts. Here, we have characterized the protein composition and N-glycome of a yeast-synthesized and commercially available whey protein ingredient and compared this novel ingredient to whey protein isolate powder derived from bovine milk. Despite strong similarities in protein composition, we found that the N-glycome significantly differs between the two protein sources, reflecting the biosynthetic machinery of the production systems. Furthermore, the diversity of proteins found in yeast-synthesized whey protein were lower relative to bovine whey protein, despite both being predominantly β-lactoglobulin. Finally, to understand whether these differences in N-glycome profiles may affect the human gut microbiome, we compared these proteins in an in vitro fecal fermentation model. The two whey protein sources generated significant differences among three representative gut microbiomes in vitro, most likely due to differences in N-glycan composition and degradation by these representative microbial communities. This work highlights the need to understand how differences in novel biotechnological systems affect the bioactivity of synthesized proteins and how these differences impact the human gut microbiome.IMPORTANCERecent advances in food technology have led to the production of animal-free products from yeast that are traditionally derived from animals, such as milk proteins. These new processes raise important questions about the use of synthetic proteins as a replacement for traditionally sourced protein, especially in the context of the gut microbiome. Importantly, yeast produce N-glycans comprised primarily of mannose, while animals synthesize structurally and compositionally complex N-glycan structures. Given these differences, we characterized a new, yeast-derived whey protein ingredient and compared it to bovine whey protein. We found that yeast-derived whey protein differs in its impact on human gut microbiomes because of differences in N-glycan structures, despite similarity in protein composition. These findings raise important questions as to whether these differences in synthetic proteins lead to significant changes to the gut microbiome in vivo, and whether this may impact the utility of these novel ingredients.https://journals.asm.org/doi/10.1128/spectrum.00200-25gut microbiomefiberN-glycanwheyprotein glycosylation
spellingShingle Matthew Bolino
Hatice Duman
İzzet Avcı
Hacı Mehmet Kayili
Juli Petereit
Chandler Zundel
Bekir Salih
Sercan Karav
Steven A. Frese
Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
Microbiology Spectrum
gut microbiome
fiber
N-glycan
whey
protein glycosylation
title Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
title_full Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
title_fullStr Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
title_full_unstemmed Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
title_short Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
title_sort proteomic and n glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes in vitro
topic gut microbiome
fiber
N-glycan
whey
protein glycosylation
url https://journals.asm.org/doi/10.1128/spectrum.00200-25
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