Non-destructive method for extracting DNA from cowpea seeds
Studies employing molecular markers require DNA extraction. For cowpea, DNA extraction is typically performed on young leaf tissue; however, this procedure requires significant time, space and resources. This study aimed to establish a protocol for extracting DNA from scarified cowpe...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2025-08-01
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| Series: | Pesquisa Agropecuária Tropical |
| Subjects: | |
| Online Access: | https://revistas.ufg.br/pat/article/view/82648/43265 |
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| author | Victoria de Oliveira Santos Eveline Nogueira Lima Ingrid Bernardo de Lima Coutinho Cândida Hermínia Campos de Magalhães Patricia do Nascimento Bordallo |
| author_facet | Victoria de Oliveira Santos Eveline Nogueira Lima Ingrid Bernardo de Lima Coutinho Cândida Hermínia Campos de Magalhães Patricia do Nascimento Bordallo |
| author_sort | Victoria de Oliveira Santos |
| collection | DOAJ |
| description | Studies employing molecular markers require DNA extraction. For cowpea, DNA extraction is typically performed on young leaf tissue; however, this procedure requires significant time, space and resources. This study aimed to establish a protocol for extracting DNA from scarified cowpea seeds while preserving the embryo viability. Three DNA extraction protocols were evaluated to determine the most suitable non-destructive method: the CTAB method, the NaOH-Tris method and a commercial kit. The commercial kit produced DNA of higher purity, when compared to the other protocols. Nevertheless, the amplified products from the commercial kit and the CTAB protocol were highly similar. Five genetic similarity groups were identified. No significant differences were observed for the germination rate or the emergence speed of scarified seeds. The genetic variability assessment validated the efficiency of the proposed methodology. This protocol effectively preserved the embryo viability, enabling the subsequent use of the seeds in genetic breeding programs. |
| format | Article |
| id | doaj-art-888d37d1e028407ebca4a7054f4b5471 |
| institution | Kabale University |
| issn | 1983-4063 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Pesquisa Agropecuária Tropical |
| spelling | doaj-art-888d37d1e028407ebca4a7054f4b54712025-08-20T18:14:04ZengUniversidade Federal de GoiásPesquisa Agropecuária Tropical1983-40632025-08-0155e8264810.1590/1983-40632025v5582648Non-destructive method for extracting DNA from cowpea seedsVictoria de Oliveira SantosEveline Nogueira LimaIngrid Bernardo de Lima CoutinhoCândida Hermínia Campos de MagalhãesPatricia do Nascimento BordalloStudies employing molecular markers require DNA extraction. For cowpea, DNA extraction is typically performed on young leaf tissue; however, this procedure requires significant time, space and resources. This study aimed to establish a protocol for extracting DNA from scarified cowpea seeds while preserving the embryo viability. Three DNA extraction protocols were evaluated to determine the most suitable non-destructive method: the CTAB method, the NaOH-Tris method and a commercial kit. The commercial kit produced DNA of higher purity, when compared to the other protocols. Nevertheless, the amplified products from the commercial kit and the CTAB protocol were highly similar. Five genetic similarity groups were identified. No significant differences were observed for the germination rate or the emergence speed of scarified seeds. The genetic variability assessment validated the efficiency of the proposed methodology. This protocol effectively preserved the embryo viability, enabling the subsequent use of the seeds in genetic breeding programs.https://revistas.ufg.br/pat/article/view/82648/43265vigna unguiculatadna extraction protocolmolecular markers |
| spellingShingle | Victoria de Oliveira Santos Eveline Nogueira Lima Ingrid Bernardo de Lima Coutinho Cândida Hermínia Campos de Magalhães Patricia do Nascimento Bordallo Non-destructive method for extracting DNA from cowpea seeds Pesquisa Agropecuária Tropical vigna unguiculata dna extraction protocol molecular markers |
| title | Non-destructive method for extracting DNA from cowpea seeds |
| title_full | Non-destructive method for extracting DNA from cowpea seeds |
| title_fullStr | Non-destructive method for extracting DNA from cowpea seeds |
| title_full_unstemmed | Non-destructive method for extracting DNA from cowpea seeds |
| title_short | Non-destructive method for extracting DNA from cowpea seeds |
| title_sort | non destructive method for extracting dna from cowpea seeds |
| topic | vigna unguiculata dna extraction protocol molecular markers |
| url | https://revistas.ufg.br/pat/article/view/82648/43265 |
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