Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis
Background and objective: In Middle Eastern countries, cutaneous leishmaniasis is still a community health concern. This study aimed to identify the Leishmania species in the local area by an accrued method using Polymerase Chain Reaction (PCR) of the mini-exon gene. Material and methods: Fourtee...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tikrit University
2025-08-01
|
| Series: | Tikrit Journal of Pure Science |
| Subjects: | |
| Online Access: | https://www.tjpsj.org/index.php/tjps/article/view/1781 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849222881326661632 |
|---|---|
| author | Evan L. Khlef Hawri M. Bakr Bejan A. Dizayee Hadi M. Alsakee |
| author_facet | Evan L. Khlef Hawri M. Bakr Bejan A. Dizayee Hadi M. Alsakee |
| author_sort | Evan L. Khlef |
| collection | DOAJ |
| description |
Background and objective: In Middle Eastern countries, cutaneous leishmaniasis is still a community health concern. This study aimed to identify the Leishmania species in the local area by an accrued method using Polymerase Chain Reaction (PCR) of the mini-exon gene.
Material and methods: Fourteen Gimsa-stained slides were collected for leishmaniasis from the private laboratory. These slides were prepared for patients with clinical manifestations of leishmaniasis. Genomic DNA was extracted using a modified Motazedian protocol. PCR technique was used to amplify all samples using specific primers for the mini-exon gene.
Results: All fourteen samples were positive for leishmaniasis by PCR amplification. Sanger sequencing has been achieved for the positive samples to identify the species. Seven samples out of 14 were identified as L. infantum, while the remaining seven samples were identified as L. major. The miniexon gene DNA data for Leishmania species (L. major and L. infantum) were submitted to the National Center for Biotechnology Information (NCBI). The sequences are given in GenBank accession numbers OP611207 (L. major) and OP611208 (L. infantum).
Conclusions: Molecular techniques such as PCR and sequencing enhance the accurate diagnosis and management of leishmaniasis.
|
| format | Article |
| id | doaj-art-888badc7a7534e70b016c85baa41e6bd |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-888badc7a7534e70b016c85baa41e6bd2025-08-26T02:38:17ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262025-08-0130410.25130/tjps.v30i4.1781 Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous LeishmaniasisEvan L. Khlef0Hawri M. Bakr 1Bejan A. Dizayee2Hadi M. Alsakee3College of Medicine/Hawler Medical University, Erbil, Iraq.College of Medicine/Hawler Medical University, Erbil, Iraq.Central Veterinary Laboratory in Kurdistan Region, Erbil, IraqCollege of Medicine, Hawler Medical University, International University of Erbil, Erbil, Iraq Background and objective: In Middle Eastern countries, cutaneous leishmaniasis is still a community health concern. This study aimed to identify the Leishmania species in the local area by an accrued method using Polymerase Chain Reaction (PCR) of the mini-exon gene. Material and methods: Fourteen Gimsa-stained slides were collected for leishmaniasis from the private laboratory. These slides were prepared for patients with clinical manifestations of leishmaniasis. Genomic DNA was extracted using a modified Motazedian protocol. PCR technique was used to amplify all samples using specific primers for the mini-exon gene. Results: All fourteen samples were positive for leishmaniasis by PCR amplification. Sanger sequencing has been achieved for the positive samples to identify the species. Seven samples out of 14 were identified as L. infantum, while the remaining seven samples were identified as L. major. The miniexon gene DNA data for Leishmania species (L. major and L. infantum) were submitted to the National Center for Biotechnology Information (NCBI). The sequences are given in GenBank accession numbers OP611207 (L. major) and OP611208 (L. infantum). Conclusions: Molecular techniques such as PCR and sequencing enhance the accurate diagnosis and management of leishmaniasis. https://www.tjpsj.org/index.php/tjps/article/view/1781Cutaneous leishmaniasis; Mini-exon gene; PCR. |
| spellingShingle | Evan L. Khlef Hawri M. Bakr Bejan A. Dizayee Hadi M. Alsakee Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis Tikrit Journal of Pure Science Cutaneous leishmaniasis; Mini-exon gene; PCR. |
| title | Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis |
| title_full | Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis |
| title_fullStr | Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis |
| title_full_unstemmed | Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis |
| title_short | Leishmania mini-exon Gene for Molecular Diagnosis and Genotypic of Cutaneous Leishmaniasis |
| title_sort | leishmania mini exon gene for molecular diagnosis and genotypic of cutaneous leishmaniasis |
| topic | Cutaneous leishmaniasis; Mini-exon gene; PCR. |
| url | https://www.tjpsj.org/index.php/tjps/article/view/1781 |
| work_keys_str_mv | AT evanlkhlef leishmaniaminiexongeneformoleculardiagnosisandgenotypicofcutaneousleishmaniasis AT hawrimbakr leishmaniaminiexongeneformoleculardiagnosisandgenotypicofcutaneousleishmaniasis AT bejanadizayee leishmaniaminiexongeneformoleculardiagnosisandgenotypicofcutaneousleishmaniasis AT hadimalsakee leishmaniaminiexongeneformoleculardiagnosisandgenotypicofcutaneousleishmaniasis |