Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae)
This study aims to establish efficient in vitro propagation protocols for <i>Gymnocalycium</i> cv. Fancy, an ornamental cactus with variegated variants, by evaluating the effects of cytokinin type and explant source on the organogenic response. Plants with different degrees of variegatio...
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MDPI AG
2025-04-01
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| Online Access: | https://www.mdpi.com/2223-7747/14/7/1091 |
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| author | Carles Cortés-Olmos Vladimir Marín Guerra-Sandoval Carla Guijarro-Real Benito Pineda Ana Fita Adrián Rodríguez-Burruezo |
| author_facet | Carles Cortés-Olmos Vladimir Marín Guerra-Sandoval Carla Guijarro-Real Benito Pineda Ana Fita Adrián Rodríguez-Burruezo |
| author_sort | Carles Cortés-Olmos |
| collection | DOAJ |
| description | This study aims to establish efficient in vitro propagation protocols for <i>Gymnocalycium</i> cv. Fancy, an ornamental cactus with variegated variants, by evaluating the effects of cytokinin type and explant source on the organogenic response. Plants with different degrees of variegation (0–100%) were classified by size to obtain different explant types (apices, central discs, epicotyls, and hypocotyls). The effects of 6-Benzylaminopurine (BAP, 8 µM), Kinetin (KIN, 4 µM), and Thidiazuron (TDZ, 1 µM) were assessed on shoot production, callus formation, and rhizogenesis. Additionally, we studied the relationship between initial plant variegation and the productivity of the variegated shoots. The best shoot production results were obtained for central discs treated with 1 µM TDZ. Furthermore, a correlation was observed between the activated areole type (green, mixed, or fully colored) and shoot color percentage, enabling precise explant selection. The appearance of differently colored shoots confirms the potential for selecting new lines from this cultivar too. These findings hold significant potential not only for the breeding and propagation of ornamental cacti but also for the cultivation of other edible cacti and their relatives. |
| format | Article |
| id | doaj-art-8872c3405e4e4a3daa488c7e4b152179 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-8872c3405e4e4a3daa488c7e4b1521792025-08-20T03:08:53ZengMDPI AGPlants2223-77472025-04-01147109110.3390/plants14071091Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae)Carles Cortés-Olmos0Vladimir Marín Guerra-Sandoval1Carla Guijarro-Real2Benito Pineda3Ana Fita4Adrián Rodríguez-Burruezo5Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, SpainInstituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, SpainDepartment Biotechnology and Plant Biology, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas ETSIAAB, Universidad Politécnica de Madrid, Av. Puerta de Hierro, 2, Moncloa-Aravaca, 28040 Madrid, SpainIBMCP, Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, SpainInstituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, SpainInstituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, SpainThis study aims to establish efficient in vitro propagation protocols for <i>Gymnocalycium</i> cv. Fancy, an ornamental cactus with variegated variants, by evaluating the effects of cytokinin type and explant source on the organogenic response. Plants with different degrees of variegation (0–100%) were classified by size to obtain different explant types (apices, central discs, epicotyls, and hypocotyls). The effects of 6-Benzylaminopurine (BAP, 8 µM), Kinetin (KIN, 4 µM), and Thidiazuron (TDZ, 1 µM) were assessed on shoot production, callus formation, and rhizogenesis. Additionally, we studied the relationship between initial plant variegation and the productivity of the variegated shoots. The best shoot production results were obtained for central discs treated with 1 µM TDZ. Furthermore, a correlation was observed between the activated areole type (green, mixed, or fully colored) and shoot color percentage, enabling precise explant selection. The appearance of differently colored shoots confirms the potential for selecting new lines from this cultivar too. These findings hold significant potential not only for the breeding and propagation of ornamental cacti but also for the cultivation of other edible cacti and their relatives.https://www.mdpi.com/2223-7747/14/7/1091citokininscolored cactusorganogenesisplant growth regulatorscactus areolasexplant |
| spellingShingle | Carles Cortés-Olmos Vladimir Marín Guerra-Sandoval Carla Guijarro-Real Benito Pineda Ana Fita Adrián Rodríguez-Burruezo Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) Plants citokinins colored cactus organogenesis plant growth regulators cactus areolas explant |
| title | Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) |
| title_full | Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) |
| title_fullStr | Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) |
| title_full_unstemmed | Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) |
| title_short | Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial <i>Gymnocalycium</i> cv. Fancy (Cactaceae) |
| title_sort | response to in vitro micropropagation of plants with different degrees of variegation of the commercial i gymnocalycium i cv fancy cactaceae |
| topic | citokinins colored cactus organogenesis plant growth regulators cactus areolas explant |
| url | https://www.mdpi.com/2223-7747/14/7/1091 |
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