Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan
Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of...
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The Journal of Infection in Developing Countries
2013-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2942 |
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| author | Adnan Khan Amber Farooqui Yasir Raza Faisal Rasheed Hamid Manzoor Syed Shakeel Akhtar Muhammad Saeed Quraishy Salvatore Rubino Shahana U Kazmi Bianca Paglietti |
| author_facet | Adnan Khan Amber Farooqui Yasir Raza Faisal Rasheed Hamid Manzoor Syed Shakeel Akhtar Muhammad Saeed Quraishy Salvatore Rubino Shahana U Kazmi Bianca Paglietti |
| author_sort | Adnan Khan |
| collection | DOAJ |
| description | Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years.
Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology.
Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC.
Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.
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| format | Article |
| id | doaj-art-88715ee81ebb44f4a6ce3b826baa3c0c |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-88715ee81ebb44f4a6ce3b826baa3c0c2025-08-20T02:57:01ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-03-0170310.3855/jidc.2942Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from PakistanAdnan Khan0Amber Farooqui1Yasir Raza2Faisal Rasheed3Hamid Manzoor4Syed Shakeel Akhtar5Muhammad Saeed Quraishy6Salvatore Rubino7Shahana U Kazmi8Bianca Paglietti9Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, PakistanDivision of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, Xinling Road 22, Shantou, Guangdong 515041Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of KarachiImmunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of KarachiDepartment of Surgery, Civil Hospital, KarachiDepartment of Surgery, Civil Hospital, KarachiDepartment of Surgery, Civil Hospital, KarachiDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyImmunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of KarachiDepartment of Biomedical Sciences, University of Sassari, Sassari,Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years. Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology. Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC. Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan. https://jidc.org/index.php/journal/article/view/2942H. pyloricagAvacAduodenal ulcergastric ulcerPakistan |
| spellingShingle | Adnan Khan Amber Farooqui Yasir Raza Faisal Rasheed Hamid Manzoor Syed Shakeel Akhtar Muhammad Saeed Quraishy Salvatore Rubino Shahana U Kazmi Bianca Paglietti Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan Journal of Infection in Developing Countries H. pylori cagA vacA duodenal ulcer gastric ulcer Pakistan |
| title | Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan |
| title_full | Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan |
| title_fullStr | Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan |
| title_full_unstemmed | Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan |
| title_short | Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan |
| title_sort | prevalence diversity and disease association of helicobacter pylori in dyspeptic patients from pakistan |
| topic | H. pylori cagA vacA duodenal ulcer gastric ulcer Pakistan |
| url | https://jidc.org/index.php/journal/article/view/2942 |
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