Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted
Hexavalent chromium (Cr(VI)) has emerged as a contaminant of heavy metal, owing to its wide use in industry. This study focuses on elucidating the interaction between microbial communities and environmental parameters in Cr(VI)-contaminated groundwater near a factory in Henan Province, and evaluatin...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015501 |
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| author | Yu Xia Yujuan Wen Yuesuo Yang Xiaoming Song Yunlong Wang Zhipeng Zhang |
| author_facet | Yu Xia Yujuan Wen Yuesuo Yang Xiaoming Song Yunlong Wang Zhipeng Zhang |
| author_sort | Yu Xia |
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| description | Hexavalent chromium (Cr(VI)) has emerged as a contaminant of heavy metal, owing to its wide use in industry. This study focuses on elucidating the interaction between microbial communities and environmental parameters in Cr(VI)-contaminated groundwater near a factory in Henan Province, and evaluating the bio-remediation potential of microorganisms toward Cr(VI) reduction. The highest concentration of Cr(VI) in the groundwater is 208.08 mg/L. The dominant microbes were Proteobacteria and Bacteroidota, closely positively related to Cr(VI) and SO42-. Many of these genus have been proven to be chromium tolerant or have the ability to reduce Cr(VI). Two strains, Micrococcus sp. HX and Bacillus sp. HX-2, were isolated from contaminated groundwater, and Micrococcus sp. HX was used for the first time to reduce Cr(VI) in groundwater. The reduced ability of HX reached 90.18 % at a Cr(VI) concentration of 100 mg/L, while HX-2 achieved a reduction capacity of 63.8 %. Micrococcus sp. HX shows the best reduction efficiency in alkaline environments (ph=8), which is close to the tannery industry wastewater. The reduction efficiency by Micrococcus sp. HX reached 67.26 % in groundwater samples (Cr(VI)= 26.08 mg/L). Transcriptome analyses revealed oxidoreductase activity, ATP binding and the NAD(P) binding region protein-related gene expression were up-regulated. Binding reduction experiments indicated that most of the Cr(III) was detected extracellular, which suggests that the reduction of Cr(VI) by HX was mainly extracellular enzyme-catalyzed. |
| format | Article |
| id | doaj-art-886e7469432348329cdde31e67c2196f |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-886e7469432348329cdde31e67c2196f2025-01-23T05:25:41ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117474Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial pollutedYu Xia0Yujuan Wen1Yuesuo Yang2Xiaoming Song3Yunlong Wang4Zhipeng Zhang5Key Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, ChinaKey Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, China; Northeast Geological S&T Innovation Center of China Geological Survey, Shenyang University, Shenyang 110044, China; Key Laboratory of Black Soil Evolution and Ecological Effect, Ministry of Natural Resources, China; Corresponding author at: Key Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, China.Key Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, China; Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Changchun 130021, ChinaKey Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, ChinaKey Laboratory of Regional Environment and Eco-restoration (Shenyang University), Ministry of Education, Shenyang University, Shenyang 110044, ChinaSichuan Geological Environment Survey and Research Center, Sichuan 610000, ChinaHexavalent chromium (Cr(VI)) has emerged as a contaminant of heavy metal, owing to its wide use in industry. This study focuses on elucidating the interaction between microbial communities and environmental parameters in Cr(VI)-contaminated groundwater near a factory in Henan Province, and evaluating the bio-remediation potential of microorganisms toward Cr(VI) reduction. The highest concentration of Cr(VI) in the groundwater is 208.08 mg/L. The dominant microbes were Proteobacteria and Bacteroidota, closely positively related to Cr(VI) and SO42-. Many of these genus have been proven to be chromium tolerant or have the ability to reduce Cr(VI). Two strains, Micrococcus sp. HX and Bacillus sp. HX-2, were isolated from contaminated groundwater, and Micrococcus sp. HX was used for the first time to reduce Cr(VI) in groundwater. The reduced ability of HX reached 90.18 % at a Cr(VI) concentration of 100 mg/L, while HX-2 achieved a reduction capacity of 63.8 %. Micrococcus sp. HX shows the best reduction efficiency in alkaline environments (ph=8), which is close to the tannery industry wastewater. The reduction efficiency by Micrococcus sp. HX reached 67.26 % in groundwater samples (Cr(VI)= 26.08 mg/L). Transcriptome analyses revealed oxidoreductase activity, ATP binding and the NAD(P) binding region protein-related gene expression were up-regulated. Binding reduction experiments indicated that most of the Cr(III) was detected extracellular, which suggests that the reduction of Cr(VI) by HX was mainly extracellular enzyme-catalyzed.http://www.sciencedirect.com/science/article/pii/S0147651324015501Cr(VI)GroundwaterMicrobial communityReductionTranscriptome |
| spellingShingle | Yu Xia Yujuan Wen Yuesuo Yang Xiaoming Song Yunlong Wang Zhipeng Zhang Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted Ecotoxicology and Environmental Safety Cr(VI) Groundwater Microbial community Reduction Transcriptome |
| title | Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted |
| title_full | Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted |
| title_fullStr | Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted |
| title_full_unstemmed | Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted |
| title_short | Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted |
| title_sort | exploring bio remediation strategies by a novel bacteria micrococcus sp strain hx in cr vi contaminated groundwater from long term industrial polluted |
| topic | Cr(VI) Groundwater Microbial community Reduction Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015501 |
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