Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion?
Background. Static cold storage (SCS) remains the standard method for organ preservation. The development of parenchymal edema during prolonged hypothermic machine perfusion (HMP) was a major barrier to the introduction of this technique for the preservation of pancreases. A short period of HMP coul...
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| Language: | English |
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Wolters Kluwer
2025-08-01
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| Series: | Transplantation Direct |
| Online Access: | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001834 |
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| author | Benoit Mesnard, MD Christophe Masset, MD, PhD Etohan Ogbemudia, MD, PhD Sarah Bruneau, PhD Mohamed Elzawahry, MD Stéphanie Le Bas-Bernardet, PhD David Minault Jeremy Hervouet Diego Cantarovich, MD, PhD Jérôme Rigaud, MD, PhD Lionel Badet, MD, PhD Peter Friend, MD, PhD Rutger Ploeg, MD, PhD Gilles Blancho, MD, PhD James Hunter, MD, PhD Thomas Prudhomme, MD Julien Branchereau, MD, PhD |
| author_facet | Benoit Mesnard, MD Christophe Masset, MD, PhD Etohan Ogbemudia, MD, PhD Sarah Bruneau, PhD Mohamed Elzawahry, MD Stéphanie Le Bas-Bernardet, PhD David Minault Jeremy Hervouet Diego Cantarovich, MD, PhD Jérôme Rigaud, MD, PhD Lionel Badet, MD, PhD Peter Friend, MD, PhD Rutger Ploeg, MD, PhD Gilles Blancho, MD, PhD James Hunter, MD, PhD Thomas Prudhomme, MD Julien Branchereau, MD, PhD |
| author_sort | Benoit Mesnard, MD |
| collection | DOAJ |
| description | Background. Static cold storage (SCS) remains the standard method for organ preservation. The development of parenchymal edema during prolonged hypothermic machine perfusion (HMP) was a major barrier to the introduction of this technique for the preservation of pancreases. A short period of HMP could optimize the pancreas for reperfusion while minimizing the side effects related to perfusion. Our objective is to evaluate the impact of short-term HMP on the pancreatic reperfusion.
Methods. A preclinical study using a controlled donation after circulatory death porcine model was conducted. After procurement, the pancreases were preserved under hypothermic conditions for 2 h either by SCS (n = 4) or HMP (n = 4). After these 2 h of preservation, the pancreases were reperfused using a normothermic machine perfusion (NMP) for 2 h. During NMP, oxygenation, perfusion parameters, biochemical analyses, a glucose stimulation insulin secretion test, and an evaluation of ischemia/reperfusion injury by photoacoustic tomography were assessed.
Results. During NMP, resistance indices were significantly lower in the HMP group compared with the SCS group, even after 2 h of reperfusion. The tissue oxygen partial pressure was higher throughout NMP after HMP preservation. Lactate and amylase levels were equal between the 2 groups. Lipase levels were higher in the HMP group. The glucose stimulation test showed no difference between the 2 groups. Photoacoustic tomography assessment showed no endothelial damage in either group.
Conclusions. Our study suggests that a short-term HMP applied to pancreases for 2 h is effective in reducing resistance indexes and improving oxygenation. |
| format | Article |
| id | doaj-art-886213785e844fdfb65e83035dde15df |
| institution | Kabale University |
| issn | 2373-8731 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Transplantation Direct |
| spelling | doaj-art-886213785e844fdfb65e83035dde15df2025-08-20T03:36:10ZengWolters KluwerTransplantation Direct2373-87312025-08-01118e183410.1097/TXD.0000000000001834202508000-00002Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion?Benoit Mesnard, MD0Christophe Masset, MD, PhD1Etohan Ogbemudia, MD, PhD2Sarah Bruneau, PhD3Mohamed Elzawahry, MD4Stéphanie Le Bas-Bernardet, PhD5David Minault6Jeremy Hervouet7Diego Cantarovich, MD, PhD8Jérôme Rigaud, MD, PhD9Lionel Badet, MD, PhD10Peter Friend, MD, PhD11Rutger Ploeg, MD, PhD12Gilles Blancho, MD, PhD13James Hunter, MD, PhD14Thomas Prudhomme, MD15Julien Branchereau, MD, PhD161 Department of Urology and Transplantation Surgery, Nantes University Hospital, Nantes, France.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.3 Nuffield Department of Surgical Science, Oxford, United Kingdom.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.3 Nuffield Department of Surgical Science, Oxford, United Kingdom.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.1 Department of Urology and Transplantation Surgery, Nantes University Hospital, Nantes, France.4 Department of Urology Surgery and Transplantation, Edouard Herriot Hospital, Lyon, France.3 Nuffield Department of Surgical Science, Oxford, United Kingdom.3 Nuffield Department of Surgical Science, Oxford, United Kingdom.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.3 Nuffield Department of Surgical Science, Oxford, United Kingdom.2 Nantes Université, CHU Nantes1, INSERM, Centre for Research in Transplantation and Translational Immunology, UMR 1064, ITUN5, Nantes, France.1 Department of Urology and Transplantation Surgery, Nantes University Hospital, Nantes, France.Background. Static cold storage (SCS) remains the standard method for organ preservation. The development of parenchymal edema during prolonged hypothermic machine perfusion (HMP) was a major barrier to the introduction of this technique for the preservation of pancreases. A short period of HMP could optimize the pancreas for reperfusion while minimizing the side effects related to perfusion. Our objective is to evaluate the impact of short-term HMP on the pancreatic reperfusion. Methods. A preclinical study using a controlled donation after circulatory death porcine model was conducted. After procurement, the pancreases were preserved under hypothermic conditions for 2 h either by SCS (n = 4) or HMP (n = 4). After these 2 h of preservation, the pancreases were reperfused using a normothermic machine perfusion (NMP) for 2 h. During NMP, oxygenation, perfusion parameters, biochemical analyses, a glucose stimulation insulin secretion test, and an evaluation of ischemia/reperfusion injury by photoacoustic tomography were assessed. Results. During NMP, resistance indices were significantly lower in the HMP group compared with the SCS group, even after 2 h of reperfusion. The tissue oxygen partial pressure was higher throughout NMP after HMP preservation. Lactate and amylase levels were equal between the 2 groups. Lipase levels were higher in the HMP group. The glucose stimulation test showed no difference between the 2 groups. Photoacoustic tomography assessment showed no endothelial damage in either group. Conclusions. Our study suggests that a short-term HMP applied to pancreases for 2 h is effective in reducing resistance indexes and improving oxygenation.http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001834 |
| spellingShingle | Benoit Mesnard, MD Christophe Masset, MD, PhD Etohan Ogbemudia, MD, PhD Sarah Bruneau, PhD Mohamed Elzawahry, MD Stéphanie Le Bas-Bernardet, PhD David Minault Jeremy Hervouet Diego Cantarovich, MD, PhD Jérôme Rigaud, MD, PhD Lionel Badet, MD, PhD Peter Friend, MD, PhD Rutger Ploeg, MD, PhD Gilles Blancho, MD, PhD James Hunter, MD, PhD Thomas Prudhomme, MD Julien Branchereau, MD, PhD Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? Transplantation Direct |
| title | Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? |
| title_full | Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? |
| title_fullStr | Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? |
| title_full_unstemmed | Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? |
| title_short | Is 2 h of Hypothermic Machine Perfusion for Pancreas Preservation Effective in Improving Graft Reperfusion? |
| title_sort | is 2 h of hypothermic machine perfusion for pancreas preservation effective in improving graft reperfusion |
| url | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001834 |
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