Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2011/129134 |
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| author | Li-Jin Cui Chen Zhang Ting Zhang Rou-Jian Lu Zheng-De Xie Ling-Lin Zhang Chuan-Yan Liu Wei-Min Zhou Li Ruan Xue-Jun Ma Wen-Jie Tan |
| author_facet | Li-Jin Cui Chen Zhang Ting Zhang Rou-Jian Lu Zheng-De Xie Ling-Lin Zhang Chuan-Yan Liu Wei-Min Zhou Li Ruan Xue-Jun Ma Wen-Jie Tan |
| author_sort | Li-Jin Cui |
| collection | DOAJ |
| description | The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation. |
| format | Article |
| id | doaj-art-8861df669fe540f2884f3a5fbfb6b4a3 |
| institution | OA Journals |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-8861df669fe540f2884f3a5fbfb6b4a32025-08-20T02:09:11ZengWileyAdvances in Virology1687-86391687-86472011-01-01201110.1155/2011/129134129134Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, ChinaLi-Jin Cui0Chen Zhang1Ting Zhang2Rou-Jian Lu3Zheng-De Xie4Ling-Lin Zhang5Chuan-Yan Liu6Wei-Min Zhou7Li Ruan8Xue-Jun Ma9Wen-Jie Tan10National Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaVirology Laboratory, Beijing Children’s Hospital, Beijing 100045, ChinaVirology Laboratory, Beijing Children’s Hospital, Beijing 100045, ChinaVirology Laboratory, Beijing Children’s Hospital, Beijing 100045, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaNational Key Laboratory of Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, ChinaThe human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation.http://dx.doi.org/10.1155/2011/129134 |
| spellingShingle | Li-Jin Cui Chen Zhang Ting Zhang Rou-Jian Lu Zheng-De Xie Ling-Lin Zhang Chuan-Yan Liu Wei-Min Zhou Li Ruan Xue-Jun Ma Wen-Jie Tan Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China Advances in Virology |
| title | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
| title_full | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
| title_fullStr | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
| title_full_unstemmed | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
| title_short | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
| title_sort | human coronaviruses hcov nl63 and hcov hku1 in hospitalized children with acute respiratory infections in beijing china |
| url | http://dx.doi.org/10.1155/2011/129134 |
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