Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods
Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb. (DTTP). The influences of 4 extraction methods, namely hot water extraction (W), acidic extraction (HA), hot-compressed water extraction (HCW) and enzyme-assisted extraction (EAE), on the...
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Tsinghua University Press
2023-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001380 |
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| author | Zuowei Zhao Li Wang Yuan Ruan Chunnan Wen Menghuan Ge Yanyan Qian Bingji Ma |
| author_facet | Zuowei Zhao Li Wang Yuan Ruan Chunnan Wen Menghuan Ge Yanyan Qian Bingji Ma |
| author_sort | Zuowei Zhao |
| collection | DOAJ |
| description | Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb. (DTTP). The influences of 4 extraction methods, namely hot water extraction (W), acidic extraction (HA), hot-compressed water extraction (HCW) and enzyme-assisted extraction (EAE), on the yields, physicochemical properties, hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared. Among these DTTP polysaccharides, DTTP-HA was outstanding in several respects. DTTP-HA was the most water soluble; it had the highest total carbohydrate content (85.08 %), the highest uronic acid (13.20 %) and the highest thermal stability. DTTP-HA and DTTP-W possessed a triple-helix structure. These 4 kinds of polysaccharides have the same types of monosaccharides, but in different molar percentages. Extraction method had a significant impact on the microstructures of the extracted polysaccharides. DTT-HA exhibited irregular structure with many holes. Among the 4 extracted methods, the DTTP-HA and DTTP-W initially exhibited higher hypoglycemic and antioxidant activities. The better bioactivities of DTTP-HA may be related to the above factors. The findings indicated that acid extraction is an effective method to extract polysaccharides with high biological activities from DTTP. |
| format | Article |
| id | doaj-art-885216ceeaf1464fa8445cb79d5b9514 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-885216ceeaf1464fa8445cb79d5b95142025-02-03T05:14:34ZengTsinghua University PressFood Science and Human Wellness2213-45302023-01-01121130139Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methodsZuowei Zhao0Li Wang1Yuan Ruan2Chunnan Wen3Menghuan Ge4Yanyan Qian5Bingji Ma6Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaCorresponding authors at: Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, China; Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaDepartment of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaDepartment of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaDepartment of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaDepartment of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaCorresponding authors at: Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, China; Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, ChinaPolysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb. (DTTP). The influences of 4 extraction methods, namely hot water extraction (W), acidic extraction (HA), hot-compressed water extraction (HCW) and enzyme-assisted extraction (EAE), on the yields, physicochemical properties, hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared. Among these DTTP polysaccharides, DTTP-HA was outstanding in several respects. DTTP-HA was the most water soluble; it had the highest total carbohydrate content (85.08 %), the highest uronic acid (13.20 %) and the highest thermal stability. DTTP-HA and DTTP-W possessed a triple-helix structure. These 4 kinds of polysaccharides have the same types of monosaccharides, but in different molar percentages. Extraction method had a significant impact on the microstructures of the extracted polysaccharides. DTT-HA exhibited irregular structure with many holes. Among the 4 extracted methods, the DTTP-HA and DTTP-W initially exhibited higher hypoglycemic and antioxidant activities. The better bioactivities of DTTP-HA may be related to the above factors. The findings indicated that acid extraction is an effective method to extract polysaccharides with high biological activities from DTTP.http://www.sciencedirect.com/science/article/pii/S2213453022001380Dioscorea opposita Thunb peelPolysaccharidesDifferent extraction methodsPhysicochemical characteristicsBiological activities |
| spellingShingle | Zuowei Zhao Li Wang Yuan Ruan Chunnan Wen Menghuan Ge Yanyan Qian Bingji Ma Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods Food Science and Human Wellness Dioscorea opposita Thunb peel Polysaccharides Different extraction methods Physicochemical characteristics Biological activities |
| title | Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods |
| title_full | Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods |
| title_fullStr | Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods |
| title_full_unstemmed | Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods |
| title_short | Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods |
| title_sort | physicochemical properties and biological activities of polysaccharides from the peel of dioscorea opposita thunb extracted by four different methods |
| topic | Dioscorea opposita Thunb peel Polysaccharides Different extraction methods Physicochemical characteristics Biological activities |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001380 |
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