Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats
Schwann cell (SC) transplantation exhibits significant potential for spinal cord injury (SCI) repair and its use as a therapeutic modality has now progressed to clinical trials for subacute and chronic human SCI. Although SC implants provide a receptive environment for axonal regrowth and support fu...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/186385 |
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| author | J. Bryan Iorgulescu Samik P. Patel Jack Louro Christian M. Andrade Andre R. Sanchez Damien D. Pearse |
| author_facet | J. Bryan Iorgulescu Samik P. Patel Jack Louro Christian M. Andrade Andre R. Sanchez Damien D. Pearse |
| author_sort | J. Bryan Iorgulescu |
| collection | DOAJ |
| description | Schwann cell (SC) transplantation exhibits significant potential for spinal cord injury (SCI) repair and its use as a therapeutic modality has now progressed to clinical trials for subacute and chronic human SCI. Although SC implants provide a receptive environment for axonal regrowth and support functional recovery in a number of experimental SCI models, axonal regeneration is largely limited to local systems and the behavioral improvements are modest without additional combinatory approaches. In the current study we investigated whether the concurrent delivery of the polyamine putrescine, started either 30 min or 1 week after SCI, could enhance the efficacy of SCs when implanted subacutely (1 week after injury) into the contused rat spinal cord. Polyamines are ubiquitous organic cations that play an important role in the regulation of the cell cycle, cell division, cytoskeletal organization, and cell differentiation. We show that the combination of putrescine with SCs provides a significant increase in implant size, an enhancement in axonal (sensory and serotonergic) sparing and/or growth, and improved open field locomotion after SCI, as compared to SC implantation alone. These findings demonstrate that polyamine supplementation can augment the effectiveness of SCs when used as a therapeutic approach for subacute SCI repair. |
| format | Article |
| id | doaj-art-885147f9a593409e8eaff98fb3d839f1 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-885147f9a593409e8eaff98fb3d839f12025-02-03T05:51:19ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/186385186385Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured RatsJ. Bryan Iorgulescu0Samik P. Patel1Jack Louro2Christian M. Andrade3Andre R. Sanchez4Damien D. Pearse5The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USAThe Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USAThe Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USAThe Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USAThe Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USAThe Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USASchwann cell (SC) transplantation exhibits significant potential for spinal cord injury (SCI) repair and its use as a therapeutic modality has now progressed to clinical trials for subacute and chronic human SCI. Although SC implants provide a receptive environment for axonal regrowth and support functional recovery in a number of experimental SCI models, axonal regeneration is largely limited to local systems and the behavioral improvements are modest without additional combinatory approaches. In the current study we investigated whether the concurrent delivery of the polyamine putrescine, started either 30 min or 1 week after SCI, could enhance the efficacy of SCs when implanted subacutely (1 week after injury) into the contused rat spinal cord. Polyamines are ubiquitous organic cations that play an important role in the regulation of the cell cycle, cell division, cytoskeletal organization, and cell differentiation. We show that the combination of putrescine with SCs provides a significant increase in implant size, an enhancement in axonal (sensory and serotonergic) sparing and/or growth, and improved open field locomotion after SCI, as compared to SC implantation alone. These findings demonstrate that polyamine supplementation can augment the effectiveness of SCs when used as a therapeutic approach for subacute SCI repair.http://dx.doi.org/10.1155/2015/186385 |
| spellingShingle | J. Bryan Iorgulescu Samik P. Patel Jack Louro Christian M. Andrade Andre R. Sanchez Damien D. Pearse Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats Neural Plasticity |
| title | Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats |
| title_full | Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats |
| title_fullStr | Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats |
| title_full_unstemmed | Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats |
| title_short | Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats |
| title_sort | acute putrescine supplementation with schwann cell implantation improves sensory and serotonergic axon growth and functional recovery in spinal cord injured rats |
| url | http://dx.doi.org/10.1155/2015/186385 |
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