OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice
Drought and salt stresses, the major environmental abiotic stresses in agriculture worldwide, affect plant growth, crop productivity, and quality. Therefore, developing crops with higher drought and salt tolerance is highly desirable. This study reported the isolation, biological function, and molec...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2023-02-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001551 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849251419241054208 |
|---|---|
| author | Fei-bing WANG Chen-zhong WAN Hao-fei NIU Ming-yang QI Gang LI Fan ZHANG Lai-bao HU Yu-xiu YE Zun-xin WANG Bao-lei PEI Xin-hong CHEN Cai-yong YUAN |
| author_facet | Fei-bing WANG Chen-zhong WAN Hao-fei NIU Ming-yang QI Gang LI Fan ZHANG Lai-bao HU Yu-xiu YE Zun-xin WANG Bao-lei PEI Xin-hong CHEN Cai-yong YUAN |
| author_sort | Fei-bing WANG |
| collection | DOAJ |
| description | Drought and salt stresses, the major environmental abiotic stresses in agriculture worldwide, affect plant growth, crop productivity, and quality. Therefore, developing crops with higher drought and salt tolerance is highly desirable. This study reported the isolation, biological function, and molecular characterization of a novel maspardin gene, OsMas1, from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl, and abscisic acid (ABA) treatments in rice. The OsMas1 gene was introduced into the rice cultivar Zhonghua 11 (wild type, WT). OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance; in contrast, OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitivity, while OsMas1-RNAi plants showed less sensitivity to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ contents and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and photosynthesis activities were significantly increased. In contrast, malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-·), and Na+ contents were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, reactive oxygen species (ROS)-scavenging system, photosynthesis, and ion transport under salt and drought stresses. Our results indicate that the OsMas1 gene improves salt and drought tolerance in rice, which may serve as a candidate gene for enhancing crop resistance to abiotic stresses. |
| format | Article |
| id | doaj-art-883e64aa43ea415ca303f16b061a3464 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-883e64aa43ea415ca303f16b061a34642025-08-20T03:56:58ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-02-0122234135910.1016/j.jia.2022.08.077OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in riceFei-bing WANG0Chen-zhong WAN1Hao-fei NIU2Ming-yang QI3Gang LI4Fan ZHANG5Lai-bao HU6Yu-xiu YE7Zun-xin WANG8Bao-lei PEI9Xin-hong CHEN10Cai-yong YUAN11School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.China; Correspondence WANG Fei-bingSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region, Huai'an 223001, P.R.ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, P.R.ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region, Huai'an 223001, P.R.ChinaDrought and salt stresses, the major environmental abiotic stresses in agriculture worldwide, affect plant growth, crop productivity, and quality. Therefore, developing crops with higher drought and salt tolerance is highly desirable. This study reported the isolation, biological function, and molecular characterization of a novel maspardin gene, OsMas1, from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl, and abscisic acid (ABA) treatments in rice. The OsMas1 gene was introduced into the rice cultivar Zhonghua 11 (wild type, WT). OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance; in contrast, OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitivity, while OsMas1-RNAi plants showed less sensitivity to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ contents and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and photosynthesis activities were significantly increased. In contrast, malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-·), and Na+ contents were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, reactive oxygen species (ROS)-scavenging system, photosynthesis, and ion transport under salt and drought stresses. Our results indicate that the OsMas1 gene improves salt and drought tolerance in rice, which may serve as a candidate gene for enhancing crop resistance to abiotic stresses.http://www.sciencedirect.com/science/article/pii/S2095311922001551ABA signalingOsMas1 genericesalt and drought tolerance |
| spellingShingle | Fei-bing WANG Chen-zhong WAN Hao-fei NIU Ming-yang QI Gang LI Fan ZHANG Lai-bao HU Yu-xiu YE Zun-xin WANG Bao-lei PEI Xin-hong CHEN Cai-yong YUAN OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice Journal of Integrative Agriculture ABA signaling OsMas1 gene rice salt and drought tolerance |
| title | OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice |
| title_full | OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice |
| title_fullStr | OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice |
| title_full_unstemmed | OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice |
| title_short | OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice |
| title_sort | osmas1 a novel maspardin protein gene confers tolerance to salt and drought stresses by regulating aba signaling in rice |
| topic | ABA signaling OsMas1 gene rice salt and drought tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001551 |
| work_keys_str_mv | AT feibingwang osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT chenzhongwan osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT haofeiniu osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT mingyangqi osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT gangli osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT fanzhang osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT laibaohu osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT yuxiuye osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT zunxinwang osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT baoleipei osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT xinhongchen osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice AT caiyongyuan osmas1anovelmaspardinproteingeneconferstolerancetosaltanddroughtstressesbyregulatingabasignalinginrice |