The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging
Background. Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8878370 |
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| author | Feng Qin Jiuzuo Huang Wenchao Zhang Mingzi Zhang Zhenjiang Li Loubin Si Xiao Long Xiaojun Wang |
| author_facet | Feng Qin Jiuzuo Huang Wenchao Zhang Mingzi Zhang Zhenjiang Li Loubin Si Xiao Long Xiaojun Wang |
| author_sort | Feng Qin |
| collection | DOAJ |
| description | Background. Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB exposure. Methods. ASCs were cocultured with UVB-irradiated and nonirradiated HDFs. We compared HDF senescence, proliferation, migration, oxidative stress, and cytokine expression. In a nude mouse UVB-induced photoaging model, ASCs were injected subcutaneously, and skin samples were collected weekly between postoperative weeks 3 through 7. Histological analysis, PCR, ELISA, and immunohistochemistry were used to analyze the effect of ASCs. Results. Compared with UVB-irradiated HDFs, nonirradiated HDFs showed higher proliferation and migration, reduced apoptosis, and fewer senescent cells when cocultured with ASCs. The expression of extracellular matrix-related cytokines was also regulated by ASCs. In addition, ASCs effectively reversed UVB-induced skin photoaging in vivo. We propose that ASCs more robustly coordinate healthy HDFs than UVB-damaged HDFs to repair aging skin. Conclusions. ASCs improved the function of both UVB-damaged and healthy HDFs through paracrine effects. However, the impact of ASCs on healthy HDFs was greater than UVB-damaged HDFs. These findings help to elucidate the underlying mechanisms of the skin rejuvenation effect of ASCs. |
| format | Article |
| id | doaj-art-883e4100df994a59ba33fc197943b583 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-883e4100df994a59ba33fc197943b5832025-08-20T02:09:31ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88783708878370The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin AgingFeng Qin0Jiuzuo Huang1Wenchao Zhang2Mingzi Zhang3Zhenjiang Li4Loubin Si5Xiao Long6Xiaojun Wang7Department of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Vascular Surgery, The First Affiliated Hospital of the Medical School of Zhejiang University, Hangzhou 310000, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100032, ChinaBackground. Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB exposure. Methods. ASCs were cocultured with UVB-irradiated and nonirradiated HDFs. We compared HDF senescence, proliferation, migration, oxidative stress, and cytokine expression. In a nude mouse UVB-induced photoaging model, ASCs were injected subcutaneously, and skin samples were collected weekly between postoperative weeks 3 through 7. Histological analysis, PCR, ELISA, and immunohistochemistry were used to analyze the effect of ASCs. Results. Compared with UVB-irradiated HDFs, nonirradiated HDFs showed higher proliferation and migration, reduced apoptosis, and fewer senescent cells when cocultured with ASCs. The expression of extracellular matrix-related cytokines was also regulated by ASCs. In addition, ASCs effectively reversed UVB-induced skin photoaging in vivo. We propose that ASCs more robustly coordinate healthy HDFs than UVB-damaged HDFs to repair aging skin. Conclusions. ASCs improved the function of both UVB-damaged and healthy HDFs through paracrine effects. However, the impact of ASCs on healthy HDFs was greater than UVB-damaged HDFs. These findings help to elucidate the underlying mechanisms of the skin rejuvenation effect of ASCs.http://dx.doi.org/10.1155/2020/8878370 |
| spellingShingle | Feng Qin Jiuzuo Huang Wenchao Zhang Mingzi Zhang Zhenjiang Li Loubin Si Xiao Long Xiaojun Wang The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging Stem Cells International |
| title | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging |
| title_full | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging |
| title_fullStr | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging |
| title_full_unstemmed | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging |
| title_short | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging |
| title_sort | paracrine effect of adipose derived stem cells orchestrates competition between different damaged dermal fibroblasts to repair uvb induced skin aging |
| url | http://dx.doi.org/10.1155/2020/8878370 |
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