Wood-Inhabiting Nematode, <i>Bursaphelenchus ussuriensis</i> sp. n. (Nematoda: Aphelenchoididae) from David Elm, with Molecular Phylogeny of the Genus Based on Partial Mitochondrial Genomes

Wood-Inhabiting Nematode, <i>Bursaphelenchus ussuriensis</i> sp. n. (Nematoda: Aphelenchoididae) from David Elm, with Molecular Phylogeny of the Genus Based on Partial Mitochondrial Genomes

A new nematode species, <i>Bursaphelenchus ussuriensis</i> sp. n. is described in the bark beetle–elm tree association (<i>Scolytus jacobsoni</i> and <i>Ulmus davidiana</i> var. <i>japonica f. suberosa</i>) in the Asian Pacific region of Russia. The ne...

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Bibliographic Details
Main Authors:	Alexander Yu. Ryss, Sergio Álvarez-Ortega, Boris D. Efeykin, Ivan A. Kerchev, Kristina S. Polyanina, Anna I. Solovyeva, Sergei A. Subbotin
Format:	Article
Language:	English
Published:	MDPI AG 2024-12-01
Series:	Plants
Subjects:	28S rRNA gene <i>Bursaphelenchus</i> spp. ITS rRNA gene mitochondrial genomes new species phylogeny
Online Access:	https://www.mdpi.com/2223-7747/14/1/93
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Summary:	A new nematode species, <i>Bursaphelenchus ussuriensis</i> sp. n. is described in the bark beetle–elm tree association (<i>Scolytus jacobsoni</i> and <i>Ulmus davidiana</i> var. <i>japonica f. suberosa</i>) in the Asian Pacific region of Russia. The new species belongs to the <i>Hofmanni</i> group of <i>Bursaphelenchus</i> and is closest to <i>B. ulmophilus</i>. Its characteristics are as follows: lateral field with three incisures, body length 497–771 µm, post-uterine sac 3.6–5.4 times vulval body diam, 56 (39–66)% of vulva–anus distance, and spicule length 10.3 (9.5–12.5 µm). The new species differs from all species of the <i>Hofmanni</i> group in the closely situated P3 and P4 male caudal papillae and the GP5 small ‘glandpapillae’ pair on the butterfly-like papillae plate, in the set of P1, P2, P3, P4, GP5; vs. in all other species, the P4 papillae pair is absent in the pattern of P1, P2, P3, GP5. The phylogenetic position of <i>B. ussuriensis</i> sp. n. with other species of the <i>Hofmanni</i> group were reconstructed using the D2–D3 expansion segments of 28S and ITS rRNA gene sequence analysis. Sequences of twelve mitochondrial protein-coding genes of <i>B. cocophilus, B. fraudulentus, B. michalskii, B. ussurensis</i> sp. n., and <i>B. willibaldi</i> were obtained in this study. Phylogenetic relationships among eighteen <i>Bursaphelenchus</i> species based on the analysis of the mtDNA sequence dataset are provided and discussed. A modified diagnosis of the <i>Hofmanni</i> group is proposed.
ISSN:	2223-7747