The Rhizobacterium <i>Bacillus amyloliquefaciens</i> MHR24 Has Biocontrol Ability Against Fungal Phytopathogens and Promotes Growth in <i>Arabidopsis thaliana</i>

The Rhizobacterium <i>Bacillus amyloliquefaciens</i> MHR24 Has Biocontrol Ability Against Fungal Phytopathogens and Promotes Growth in <i>Arabidopsis thaliana</i>

A novel rhizobacteria <i>Bacillus</i> was isolated from rhizosphere of soil associated with tomato (<i>Solanum lycopersicum</i> L.) under open field conditions. The <i>Bacillus amyloliquefaciens</i> strain MHR24 (MHR24) is a promising biocontrol agent against seve...

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Main Authors: Mónica Hernández-Rodríguez, Diana Jasso-de Rodríguez, Francisco Daniel Hernández-Castillo, Ivana Moggio, Eduardo Arias, José Humberto Valenzuela-Soto, Alberto Flores-Olivas
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Microorganisms
Subjects:
biocontrol agent
<i>Bacillus amyloliquefaciens</i>
fungal inhibition
plant growth promotion
<i>Arabidopsis thaliana</i>
Online Access:https://www.mdpi.com/2076-2607/12/11/2380
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Summary:A novel rhizobacteria <i>Bacillus</i> was isolated from rhizosphere of soil associated with tomato (<i>Solanum lycopersicum</i> L.) under open field conditions. The <i>Bacillus amyloliquefaciens</i> strain MHR24 (MHR24) is a promising biocontrol agent against several fungal phytopathogens. In this research, MHR24 was characterized by an effective antagonistic ability against <i>Alternaria alternata</i> (Aa), <i>Botrytis cinerea</i> (Bc), <i>Fusarium oxysporum</i> F1 (F1), <i>F. oxysporum</i> F2 (F2), <i>F. oxysporum</i> R3 (F3), and <i>Sclerotinia sclerotiorum</i> (Sc). In particular, MHR24 showed a strong inhibition via airborne volatiles against Bc, F3, Aa, and F2 fungal strains. MHR24 also showed elevated saline stress tolerance at 1% and 25% to NaCl and KCl. The molecular sequence analysis of 16S rDNA confirmed the identity of the isolate as <i>Bacillus amyloliquefaciens</i> strain MHR24. Bioassays on <i>Arabidopsis thaliana</i> Col-0 inoculated with MHR24 showed in in vitro conditions that MHR24 significantly increases the foliar and root area, while in growth chamber conditions, it strongly increases the dry shoot biomass of <i>A. thaliana</i>. The observed results indicate that <i>B. amyloliquefaciens</i> MHR24 has a broad-spectrum biocontrol against fungal phytopathogens and can be used as a biofertilizer and biocontrol agent to improve horticultural crops.
ISSN:2076-2607

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