Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections
<i>Klebsiella pneumoniae</i> is a Gram-negative bacterium commonly associated with bloodstream infections (BSIs), which can lead to severe clinical outcomes, especially in immunocompromised individuals or patients with underlying health conditions. The increasing prevalence of <i>K...
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2025-02-01
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| author | Carolina Sabença Rani Rivière Eliana Costa Sara Sousa Manuela Caniça Vanessa Silva Gilberto Igrejas Carmen Torres Patrícia Poeta |
| author_facet | Carolina Sabença Rani Rivière Eliana Costa Sara Sousa Manuela Caniça Vanessa Silva Gilberto Igrejas Carmen Torres Patrícia Poeta |
| author_sort | Carolina Sabença |
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| description | <i>Klebsiella pneumoniae</i> is a Gram-negative bacterium commonly associated with bloodstream infections (BSIs), which can lead to severe clinical outcomes, especially in immunocompromised individuals or patients with underlying health conditions. The increasing prevalence of <i>K. pneumoniae</i> that produces extended-spectrum β-lactamases (ESBL) poses a significant challenge for treatment and infection control, necessitating a swift diagnostic approach and tailored antimicrobial therapy to improve patient outcomes. A total of 32 <i>K. pneumoniae</i> isolates were recovered from BSIs from December 2021 to August 2022. Whole-genome sequencing (WGS) was performed on the 14 ESBL-producing isolates. All ESBL isolates carried the <i>bla</i><sub>CTX-M-15</sub> gene, together with other β-lactamase-encoding genes (<i>bla</i><sub>TEM-1</sub>, <i>bla</i><sub>SHV-28</sub>, <i>bla</i><sub>SHV-26</sub>, or <i>bla</i><sub>OXA-1</sub>). Three of the isolates also carried the <i>bla</i><sub>KPC-3</sub> gene. Resistance genes to quinolones, sulfonamides, tetracycline, aminoglycosides, and chloramphenicol were also detected. We can conclude that the presence of ESBL-producing isolates among <i>K. pneumoniae</i> of BSIs raises concerns, since these enzymes limit the available treatment options, and future research must include studies on alternative therapies for dealing with resistant bacterial infections and developing new approaches to disease treatment. |
| format | Article |
| id | doaj-art-882dc68907974a6985b36ec283439f7a |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
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| series | Pathogens |
| spelling | doaj-art-882dc68907974a6985b36ec283439f7a2025-08-20T03:43:31ZengMDPI AGPathogens2076-08172025-02-0114320510.3390/pathogens14030205Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream InfectionsCarolina Sabença0Rani Rivière1Eliana Costa2Sara Sousa3Manuela Caniça4Vanessa Silva5Gilberto Igrejas6Carmen Torres7Patrícia Poeta8MicroART-Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalHospital Centre of Trás-os-Montes and Alto Douro, Clinical Pathology Department, 5000-508 Vila Real, PortugalHospital Centre of Trás-os-Montes and Alto Douro, Clinical Pathology Department, 5000-508 Vila Real, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalMicroART-Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalArea Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, SpainMicroART-Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal<i>Klebsiella pneumoniae</i> is a Gram-negative bacterium commonly associated with bloodstream infections (BSIs), which can lead to severe clinical outcomes, especially in immunocompromised individuals or patients with underlying health conditions. The increasing prevalence of <i>K. pneumoniae</i> that produces extended-spectrum β-lactamases (ESBL) poses a significant challenge for treatment and infection control, necessitating a swift diagnostic approach and tailored antimicrobial therapy to improve patient outcomes. A total of 32 <i>K. pneumoniae</i> isolates were recovered from BSIs from December 2021 to August 2022. Whole-genome sequencing (WGS) was performed on the 14 ESBL-producing isolates. All ESBL isolates carried the <i>bla</i><sub>CTX-M-15</sub> gene, together with other β-lactamase-encoding genes (<i>bla</i><sub>TEM-1</sub>, <i>bla</i><sub>SHV-28</sub>, <i>bla</i><sub>SHV-26</sub>, or <i>bla</i><sub>OXA-1</sub>). Three of the isolates also carried the <i>bla</i><sub>KPC-3</sub> gene. Resistance genes to quinolones, sulfonamides, tetracycline, aminoglycosides, and chloramphenicol were also detected. We can conclude that the presence of ESBL-producing isolates among <i>K. pneumoniae</i> of BSIs raises concerns, since these enzymes limit the available treatment options, and future research must include studies on alternative therapies for dealing with resistant bacterial infections and developing new approaches to disease treatment.https://www.mdpi.com/2076-0817/14/3/205extended-spectrum ?-lactamases<i>Klebsiella pneumoniae</i>bloodstream infectionswhole-genome sequencing |
| spellingShingle | Carolina Sabença Rani Rivière Eliana Costa Sara Sousa Manuela Caniça Vanessa Silva Gilberto Igrejas Carmen Torres Patrícia Poeta Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections Pathogens extended-spectrum ?-lactamases <i>Klebsiella pneumoniae</i> bloodstream infections whole-genome sequencing |
| title | Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections |
| title_full | Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections |
| title_fullStr | Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections |
| title_full_unstemmed | Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections |
| title_short | Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Isolated from Human Bloodstream Infections |
| title_sort | whole genome sequencing of extended spectrum β lactamase producing i klebsiella pneumoniae i isolated from human bloodstream infections |
| topic | extended-spectrum ?-lactamases <i>Klebsiella pneumoniae</i> bloodstream infections whole-genome sequencing |
| url | https://www.mdpi.com/2076-0817/14/3/205 |
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