Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study
Glyphosate, a widely used herbicide, poses environmental concerns due to its persistence and potential health hazards. In this study, we explored the efficacy of the laccase enzyme immobilized on the surface of magnetic iron oxide nanoparticles (IONPs) for the degradation of glyphosate pesticides. T...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Environmental Mutagen Society
2025-05-01
|
| Series: | Journal of Water and Environmental Nanotechnology |
| Subjects: | |
| Online Access: | https://www.jwent.net/article_724948_52dfa5b98aebea5870e16277366a7ba4.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849233855439962112 |
|---|---|
| author | Amanpreet K. Sidhu Sucheta N. Patil Vishwasrao B. Gaikwad |
| author_facet | Amanpreet K. Sidhu Sucheta N. Patil Vishwasrao B. Gaikwad |
| author_sort | Amanpreet K. Sidhu |
| collection | DOAJ |
| description | Glyphosate, a widely used herbicide, poses environmental concerns due to its persistence and potential health hazards. In this study, we explored the efficacy of the laccase enzyme immobilized on the surface of magnetic iron oxide nanoparticles (IONPs) for the degradation of glyphosate pesticides. The immobilized laccase exhibited facile recovery and reusability, offering a sustainable approach to pesticide remediation. A comparative investigation was conducted to assess the degradation efficiency of glyphosate using free laccase enzyme and two variants of laccase immobilized on IONPs, namely IONP-Thio-Lac and IONP-PVA-Lac. Optimization experiments were conducted to evaluate the impact of pesticide concentration, temperature, incubation time, and enzyme units on the degradation process. High-Performance Liquid Chromatography (HPLC) studies confirmed the efficacy of the immobilized laccase systems. Results revealed that after 1 hour, the percent degradation of a 5000 ppm glyphosate solution using free laccase, IONP-Thio-Lac, and IONP-PVA-Lac were 29.534%, 40.533%, and 74.758%, respectively. The findings underscore the effectiveness of laccase immobilization on magnetic nanoparticles for glyphosate degradation, with IONP-PVA-Lac exhibiting the highest degradation efficiency. This research highlights a promising strategy for sustainable pesticide remediation, offering potential applications in environmental protection and agriculture. |
| format | Article |
| id | doaj-art-8825a55ebc934dcf80b2b9b9ece84aaf |
| institution | Kabale University |
| issn | 2476-7204 2476-6615 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Iranian Environmental Mutagen Society |
| record_format | Article |
| series | Journal of Water and Environmental Nanotechnology |
| spelling | doaj-art-8825a55ebc934dcf80b2b9b9ece84aaf2025-08-20T04:03:22ZengIranian Environmental Mutagen SocietyJournal of Water and Environmental Nanotechnology2476-72042476-66152025-05-0110217318310.22090/jwent.2025.02.006724948Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative StudyAmanpreet K. Sidhu0Sucheta N. Patil1Vishwasrao B. Gaikwad2Department of Science, Khalsa College for Women, Amritsar, IndiaRetd. Professor, Department of Biotechnology, KTHM College, Nashik, MS, IndiaSenior Academic Consultant, Regional Center Pune, Yashwantrao Chavan Maharashtra Open University, Nashik, MS, IndiaGlyphosate, a widely used herbicide, poses environmental concerns due to its persistence and potential health hazards. In this study, we explored the efficacy of the laccase enzyme immobilized on the surface of magnetic iron oxide nanoparticles (IONPs) for the degradation of glyphosate pesticides. The immobilized laccase exhibited facile recovery and reusability, offering a sustainable approach to pesticide remediation. A comparative investigation was conducted to assess the degradation efficiency of glyphosate using free laccase enzyme and two variants of laccase immobilized on IONPs, namely IONP-Thio-Lac and IONP-PVA-Lac. Optimization experiments were conducted to evaluate the impact of pesticide concentration, temperature, incubation time, and enzyme units on the degradation process. High-Performance Liquid Chromatography (HPLC) studies confirmed the efficacy of the immobilized laccase systems. Results revealed that after 1 hour, the percent degradation of a 5000 ppm glyphosate solution using free laccase, IONP-Thio-Lac, and IONP-PVA-Lac were 29.534%, 40.533%, and 74.758%, respectively. The findings underscore the effectiveness of laccase immobilization on magnetic nanoparticles for glyphosate degradation, with IONP-PVA-Lac exhibiting the highest degradation efficiency. This research highlights a promising strategy for sustainable pesticide remediation, offering potential applications in environmental protection and agriculture.https://www.jwent.net/article_724948_52dfa5b98aebea5870e16277366a7ba4.pdfdirect bindingglyphosateimmobilizationiron oxide nanoparticleslaccase |
| spellingShingle | Amanpreet K. Sidhu Sucheta N. Patil Vishwasrao B. Gaikwad Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study Journal of Water and Environmental Nanotechnology direct binding glyphosate immobilization iron oxide nanoparticles laccase |
| title | Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study |
| title_full | Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study |
| title_fullStr | Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study |
| title_full_unstemmed | Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study |
| title_short | Enhanced Glyphosate Degradation Using Laccase-Immobilized Magnetic Iron Oxide Nanoparticles: A Comparative Study |
| title_sort | enhanced glyphosate degradation using laccase immobilized magnetic iron oxide nanoparticles a comparative study |
| topic | direct binding glyphosate immobilization iron oxide nanoparticles laccase |
| url | https://www.jwent.net/article_724948_52dfa5b98aebea5870e16277366a7ba4.pdf |
| work_keys_str_mv | AT amanpreetksidhu enhancedglyphosatedegradationusinglaccaseimmobilizedmagneticironoxidenanoparticlesacomparativestudy AT suchetanpatil enhancedglyphosatedegradationusinglaccaseimmobilizedmagneticironoxidenanoparticlesacomparativestudy AT vishwasraobgaikwad enhancedglyphosatedegradationusinglaccaseimmobilizedmagneticironoxidenanoparticlesacomparativestudy |