An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i>
Pests and diseases have caused significant problems since the domestication of crops, resulting in economic loss and hunger. To overcome these problems, synthetic pesticides were developed to control pests; however, there are significant detrimental side effects of synthetic pesticides on the enviro...
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2024-12-01
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| author | Getrude G. Kanyairita Desmond G. Mortley Willard E. Collier Sheritta Fagbodun Jamila M. Mweta Hilarie Uwamahoro Le’Shaun T. Dowell Mwamba F. Mukuka |
| author_facet | Getrude G. Kanyairita Desmond G. Mortley Willard E. Collier Sheritta Fagbodun Jamila M. Mweta Hilarie Uwamahoro Le’Shaun T. Dowell Mwamba F. Mukuka |
| author_sort | Getrude G. Kanyairita |
| collection | DOAJ |
| description | Pests and diseases have caused significant problems since the domestication of crops, resulting in economic loss and hunger. To overcome these problems, synthetic pesticides were developed to control pests; however, there are significant detrimental side effects of synthetic pesticides on the environment and human health. There is an urgent need to develop safer and more sustainable pesticides. Industrial hemp is a reservoir of compounds that could potentially replace some synthetic bactericides, fungicides, and insecticides. We determined the efficacy of industrial hemp extracts against <i>Pseudomonas syringae</i> pv. <i>tabaci</i> (PSTA), <i>Pseudomonas syringae</i> pv. <i>tomato</i> (PSTO), and <i>Erwinia carotovora</i> (EC). The study revealed a minimum inhibitory concentration (MIC) of 2.05 mg/mL and a non-inhibitory concentration (NIC) of 1.2 mg/mL for PSTA, an MIC of 5.7 mg/mL and NIC of 0.66 mg/mL for PSTO, and an MIC of 12.04 mg/mL and NIC of 5.4 mg/mL for EC. Time-kill assays indicated the regrowth of <i>E. carotovora</i> at 4 × MIC after 15 h and <i>P. syringae</i> pv. <i>tomato</i> at 2 × MIC after 20 h; however, <i>P. syringae</i> pv. <i>tabaci</i> had no regrowth. The susceptibility of test bacteria to hemp extract can be ordered from the most susceptible to the least susceptible, as follows: <i>P. syringae</i> pv. <i>tabaci</i> > <i>P. syringae</i> pv. <i>tomato</i> > <i>E. carotovora</i>. Overall, the data indicate hemp extract is a potential source of sustainable and safe biopesticides against these major plant pathogens. |
| format | Article |
| id | doaj-art-8824f187b9e640d3b0932db6dbee3beb |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Molecules |
| spelling | doaj-art-8824f187b9e640d3b0932db6dbee3beb2025-08-20T02:51:07ZengMDPI AGMolecules1420-30492024-12-012924590210.3390/molecules29245902An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i>Getrude G. Kanyairita0Desmond G. Mortley1Willard E. Collier2Sheritta Fagbodun3Jamila M. Mweta4Hilarie Uwamahoro5Le’Shaun T. Dowell6Mwamba F. Mukuka7Department of Agriculture and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USADepartment of Agriculture and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USADepartment of Chemistry, Tuskegee University, Tuskegee, AL 36088, USADepartment of Biology, Tuskegee University, Tuskegee, AL 36088, USADepartment of Agriculture and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USADepartment of Chemistry, Tuskegee University, Tuskegee, AL 36088, USADepartment of Mathematics & Computer Science, Alabama State University, Montgomery, AL 36104, USADepartment of Mathematics & Computer Science, Alabama State University, Montgomery, AL 36104, USAPests and diseases have caused significant problems since the domestication of crops, resulting in economic loss and hunger. To overcome these problems, synthetic pesticides were developed to control pests; however, there are significant detrimental side effects of synthetic pesticides on the environment and human health. There is an urgent need to develop safer and more sustainable pesticides. Industrial hemp is a reservoir of compounds that could potentially replace some synthetic bactericides, fungicides, and insecticides. We determined the efficacy of industrial hemp extracts against <i>Pseudomonas syringae</i> pv. <i>tabaci</i> (PSTA), <i>Pseudomonas syringae</i> pv. <i>tomato</i> (PSTO), and <i>Erwinia carotovora</i> (EC). The study revealed a minimum inhibitory concentration (MIC) of 2.05 mg/mL and a non-inhibitory concentration (NIC) of 1.2 mg/mL for PSTA, an MIC of 5.7 mg/mL and NIC of 0.66 mg/mL for PSTO, and an MIC of 12.04 mg/mL and NIC of 5.4 mg/mL for EC. Time-kill assays indicated the regrowth of <i>E. carotovora</i> at 4 × MIC after 15 h and <i>P. syringae</i> pv. <i>tomato</i> at 2 × MIC after 20 h; however, <i>P. syringae</i> pv. <i>tabaci</i> had no regrowth. The susceptibility of test bacteria to hemp extract can be ordered from the most susceptible to the least susceptible, as follows: <i>P. syringae</i> pv. <i>tabaci</i> > <i>P. syringae</i> pv. <i>tomato</i> > <i>E. carotovora</i>. Overall, the data indicate hemp extract is a potential source of sustainable and safe biopesticides against these major plant pathogens.https://www.mdpi.com/1420-3049/29/24/5902hemp extractErwinia carotovora<i>Pseudomonas syringae</i> pv<i>. tomato</i><i>Pseudomonas syringae</i> pv<i>. tabaci</i>antimicrobialminimum inhibitory concentration |
| spellingShingle | Getrude G. Kanyairita Desmond G. Mortley Willard E. Collier Sheritta Fagbodun Jamila M. Mweta Hilarie Uwamahoro Le’Shaun T. Dowell Mwamba F. Mukuka An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> Molecules hemp extract Erwinia carotovora <i>Pseudomonas syringae</i> pv<i>. tomato</i> <i>Pseudomonas syringae</i> pv<i>. tabaci</i> antimicrobial minimum inhibitory concentration |
| title | An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> |
| title_full | An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> |
| title_fullStr | An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> |
| title_full_unstemmed | An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> |
| title_short | An In Vitro Evaluation of Industrial Hemp Extracts Against the Phytopathogenic Bacteria <i>Erwinia carotovora</i>, <i>Pseudomonas syringae</i> pv. <i>tomato</i>, and <i>Pseudomonas syringae</i> pv. <i>tabaci</i> |
| title_sort | in vitro evaluation of industrial hemp extracts against the phytopathogenic bacteria i erwinia carotovora i i pseudomonas syringae i pv i tomato i and i pseudomonas syringae i pv i tabaci i |
| topic | hemp extract Erwinia carotovora <i>Pseudomonas syringae</i> pv<i>. tomato</i> <i>Pseudomonas syringae</i> pv<i>. tabaci</i> antimicrobial minimum inhibitory concentration |
| url | https://www.mdpi.com/1420-3049/29/24/5902 |
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