Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves

This study investigates the effects of heat stress in a climate chamber from day 0 to 28 days of life on physiological responses, intake, nutrient digestibility, immunity, and performance in neonatal Holstein calves. Thirty-four calves (nineteen females, fifteen males) were randomly assigned to a co...

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Main Authors: Luiz F. M. Neves, Mariana B. Gomes, Joana P. Campolina, Mariana M. Campos, Eduardo M. B. Souza, Jaciara Diavão, Abias S. Silva, Thierry R. Tomich, Wanessa A. Carvalho, Helena F. Lage, Sandra G. Coelho
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/13/1876
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author Luiz F. M. Neves
Mariana B. Gomes
Joana P. Campolina
Mariana M. Campos
Eduardo M. B. Souza
Jaciara Diavão
Abias S. Silva
Thierry R. Tomich
Wanessa A. Carvalho
Helena F. Lage
Sandra G. Coelho
author_facet Luiz F. M. Neves
Mariana B. Gomes
Joana P. Campolina
Mariana M. Campos
Eduardo M. B. Souza
Jaciara Diavão
Abias S. Silva
Thierry R. Tomich
Wanessa A. Carvalho
Helena F. Lage
Sandra G. Coelho
author_sort Luiz F. M. Neves
collection DOAJ
description This study investigates the effects of heat stress in a climate chamber from day 0 to 28 days of life on physiological responses, intake, nutrient digestibility, immunity, and performance in neonatal Holstein calves. Thirty-four calves (nineteen females, fifteen males) were randomly assigned to a control group (CON, temperature–humidity index [THI] 66, with 22 °C and 65% humidity for 24 h, n = 17) or a heat-stressed (HS) group, which was exposed to a THI of 82, 32 °C, and 65% humidity for 9 h, followed by a THI of 66, 22 °C, and 65% humidity for 15 h. The HS calves exhibited increased respiratory rates and rectal temperatures (<i>p</i> < 0.001), particularly during heat exposure periods, as well as a 59.5% increase in water intake compared to CON. While milk and solid feed intake, average daily gain, and feed efficiency were similar between groups, HS calves had reduced ether extract digestibility and altered ruminal fermentation, including lower acetate and lower propionate concentrations. The blood cytokine analysis showed elevated interleukin-4 and reduced interleukin-8 and IP-10 levels in heat-stressed calves, indicating a shift toward an anti-inflammatory immune profile. Despite no major performance impairments, heat stress has induced clear physiological, digestive, and immunological changes. These results underscore the importance of implementing thermal mitigation strategies during early life to safeguard calf health and development in increasingly warmer climates.
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spelling doaj-art-881adb7ff49545f79774ccc0c08957fa2025-08-20T03:17:04ZengMDPI AGAnimals2076-26152025-06-011513187610.3390/ani15131876Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy CalvesLuiz F. M. Neves0Mariana B. Gomes1Joana P. Campolina2Mariana M. Campos3Eduardo M. B. Souza4Jaciara Diavão5Abias S. Silva6Thierry R. Tomich7Wanessa A. Carvalho8Helena F. Lage9Sandra G. Coelho10Departamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilDepartamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilDepartamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilBrazilian Agricultural Research Corporation—Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, BrazilDepartamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilBrazilian Agricultural Research Corporation—Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, BrazilBrazilian Agricultural Research Corporation—Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, BrazilBrazilian Agricultural Research Corporation—Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, BrazilBrazilian Agricultural Research Corporation—Embrapa Gado de Leite, Juiz de Fora 36038-330, MG, BrazilDepartamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilDepartamento de Zootecnia, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, MG, BrazilThis study investigates the effects of heat stress in a climate chamber from day 0 to 28 days of life on physiological responses, intake, nutrient digestibility, immunity, and performance in neonatal Holstein calves. Thirty-four calves (nineteen females, fifteen males) were randomly assigned to a control group (CON, temperature–humidity index [THI] 66, with 22 °C and 65% humidity for 24 h, n = 17) or a heat-stressed (HS) group, which was exposed to a THI of 82, 32 °C, and 65% humidity for 9 h, followed by a THI of 66, 22 °C, and 65% humidity for 15 h. The HS calves exhibited increased respiratory rates and rectal temperatures (<i>p</i> < 0.001), particularly during heat exposure periods, as well as a 59.5% increase in water intake compared to CON. While milk and solid feed intake, average daily gain, and feed efficiency were similar between groups, HS calves had reduced ether extract digestibility and altered ruminal fermentation, including lower acetate and lower propionate concentrations. The blood cytokine analysis showed elevated interleukin-4 and reduced interleukin-8 and IP-10 levels in heat-stressed calves, indicating a shift toward an anti-inflammatory immune profile. Despite no major performance impairments, heat stress has induced clear physiological, digestive, and immunological changes. These results underscore the importance of implementing thermal mitigation strategies during early life to safeguard calf health and development in increasingly warmer climates.https://www.mdpi.com/2076-2615/15/13/1876ambiancedairy calfdiscomfortpre-weaning phasetemperature
spellingShingle Luiz F. M. Neves
Mariana B. Gomes
Joana P. Campolina
Mariana M. Campos
Eduardo M. B. Souza
Jaciara Diavão
Abias S. Silva
Thierry R. Tomich
Wanessa A. Carvalho
Helena F. Lage
Sandra G. Coelho
Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
Animals
ambiance
dairy calf
discomfort
pre-weaning phase
temperature
title Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
title_full Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
title_fullStr Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
title_full_unstemmed Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
title_short Impact of Heat Stress on Intake, Performance, Digestibility, and Health of Neonatal Dairy Calves
title_sort impact of heat stress on intake performance digestibility and health of neonatal dairy calves
topic ambiance
dairy calf
discomfort
pre-weaning phase
temperature
url https://www.mdpi.com/2076-2615/15/13/1876
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