Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study
Objective: The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isola...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652400448X |
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| Summary: | Objective: The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isolated from the stool of a hospitalised patient in China. Methods: A faecal sample was diluted in Luria-Bertani broth and incubated overnight. Cultures were then spread on MacConkey agar containing meropenem and incubated for 18–24 h to select carbapenem-resistant Enterobacteriaceae. Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. Genomic DNA was extracted and sequenced via Illumina and Oxford Nanopore sequencing. Results: Genomic analysis of E. roggenkampii L3897 revealed a 4 897 636 bp genome with 55.9% GC content and confirmed its classification as E. roggenkampii through average nucleotide identity-based analysis. A new sequence type distinct from ST2392, ST3014, with a unique rplB genotype was identified. Additionally, the genome harbours three antibiotic resistance genes and a newly discovered plasmid, pL3897_NDM, highlighting the need for surveillance of drug-resistant pathogens. Conclusions: The discovery of a new sequence type and the presence of an antibiotic resistance gene in E. roggenkampii L3897 underscores the need for ongoing genomic surveillance to effectively manage multidrug-resistant pathogens. |
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| ISSN: | 2213-7165 |