UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form

A simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for the determination of tadalafil in bulk and tablet dosage form. Solution of tadalafil in methanol shows maximum absorbance at 284 nm. Beer’s law was obeyed in the concentration range of 2-20 mcg mL-1...

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Main Authors: Mohammad Yunoos, D.Gowri Sankar, B.Pragati Kumar, Shahul Hameed
Format: Article
Language:English
Published: Wiley 2010-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2010/630576
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author Mohammad Yunoos
D.Gowri Sankar
B.Pragati Kumar
Shahul Hameed
author_facet Mohammad Yunoos
D.Gowri Sankar
B.Pragati Kumar
Shahul Hameed
author_sort Mohammad Yunoos
collection DOAJ
description A simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for the determination of tadalafil in bulk and tablet dosage form. Solution of tadalafil in methanol shows maximum absorbance at 284 nm. Beer’s law was obeyed in the concentration range of 2-20 mcg mL-1 with 1.65x104 mol-1 cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of tadalafil in bulk and solid dosage form.
format Article
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institution OA Journals
issn 0973-4945
2090-9810
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publishDate 2010-01-01
publisher Wiley
record_format Article
series E-Journal of Chemistry
spelling doaj-art-88102dc85ff64f599b402f25ffe104f42025-08-20T02:09:31ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017383383610.1155/2010/630576UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage formMohammad Yunoos0D.Gowri Sankar1B.Pragati Kumar2Shahul Hameed3Nimra College of pharmacy, Jupudi, Ibrahimpatnam, Vijayawada, IndiaNimra College of pharmacy, Jupudi, Ibrahimpatnam, Vijayawada, IndiaNimra College of pharmacy, Jupudi, Ibrahimpatnam, Vijayawada, IndiaNimra College of pharmacy, Jupudi, Ibrahimpatnam, Vijayawada, IndiaA simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for the determination of tadalafil in bulk and tablet dosage form. Solution of tadalafil in methanol shows maximum absorbance at 284 nm. Beer’s law was obeyed in the concentration range of 2-20 mcg mL-1 with 1.65x104 mol-1 cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of tadalafil in bulk and solid dosage form.http://dx.doi.org/10.1155/2010/630576
spellingShingle Mohammad Yunoos
D.Gowri Sankar
B.Pragati Kumar
Shahul Hameed
UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
E-Journal of Chemistry
title UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
title_full UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
title_fullStr UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
title_full_unstemmed UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
title_short UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form
title_sort uv spectrophotometric method for the estimation of tadalafil in bulk and tablet dosage form
url http://dx.doi.org/10.1155/2010/630576
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