CAR modulates E-cadherin dynamics in the presence of adenovirus type 5.
Adenovirus (Ad) serotype 5 (Ad5) fiber competitively binds to the coxsackievirus and Ad receptor (CAR) to attach Ad5 to target cells and also disrupts cell junctions and facilitates virus escape at a late stage in Ad5 infection. Here we demonstrate that paracellular permeability in MCF7 and CAR over...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023056&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849330473705144320 |
|---|---|
| author | Fawziyah Hussain Penny E Morton Marjolein Snippe Janis Sullivan Charlotte Farmer Marisa L Martin-Fernandez Maddy Parsons George Santis |
| author_facet | Fawziyah Hussain Penny E Morton Marjolein Snippe Janis Sullivan Charlotte Farmer Marisa L Martin-Fernandez Maddy Parsons George Santis |
| author_sort | Fawziyah Hussain |
| collection | DOAJ |
| description | Adenovirus (Ad) serotype 5 (Ad5) fiber competitively binds to the coxsackievirus and Ad receptor (CAR) to attach Ad5 to target cells and also disrupts cell junctions and facilitates virus escape at a late stage in Ad5 infection. Here we demonstrate that paracellular permeability in MCF7 and CAR overexpressing MCF7 (FLCARMCF7) cells is increased within minutes following the addition of Ad5 to cells. This is brought about, at least in part, by altering the molecular dynamics of E-cadherin, a key component of the cell-cell adhesion complex. We also demonstrate that the increase in E-cadherin mobility is constitutively altered by the presence of CAR at FLCARMCF7 cell junctions. As increased paracellular permeability was observed early after the addition of Ad5 to cells, we postulate that this may represent a mechanism by which Ad5 could disrupt cell junctions to facilitate further access to its cell receptors. |
| format | Article |
| id | doaj-art-87fbf88325aa4f7e96b8b26a59945aa5 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-87fbf88325aa4f7e96b8b26a59945aa52025-08-20T03:46:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2305610.1371/journal.pone.0023056CAR modulates E-cadherin dynamics in the presence of adenovirus type 5.Fawziyah HussainPenny E MortonMarjolein SnippeJanis SullivanCharlotte FarmerMarisa L Martin-FernandezMaddy ParsonsGeorge SantisAdenovirus (Ad) serotype 5 (Ad5) fiber competitively binds to the coxsackievirus and Ad receptor (CAR) to attach Ad5 to target cells and also disrupts cell junctions and facilitates virus escape at a late stage in Ad5 infection. Here we demonstrate that paracellular permeability in MCF7 and CAR overexpressing MCF7 (FLCARMCF7) cells is increased within minutes following the addition of Ad5 to cells. This is brought about, at least in part, by altering the molecular dynamics of E-cadherin, a key component of the cell-cell adhesion complex. We also demonstrate that the increase in E-cadherin mobility is constitutively altered by the presence of CAR at FLCARMCF7 cell junctions. As increased paracellular permeability was observed early after the addition of Ad5 to cells, we postulate that this may represent a mechanism by which Ad5 could disrupt cell junctions to facilitate further access to its cell receptors.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023056&type=printable |
| spellingShingle | Fawziyah Hussain Penny E Morton Marjolein Snippe Janis Sullivan Charlotte Farmer Marisa L Martin-Fernandez Maddy Parsons George Santis CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. PLoS ONE |
| title | CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. |
| title_full | CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. |
| title_fullStr | CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. |
| title_full_unstemmed | CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. |
| title_short | CAR modulates E-cadherin dynamics in the presence of adenovirus type 5. |
| title_sort | car modulates e cadherin dynamics in the presence of adenovirus type 5 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023056&type=printable |
| work_keys_str_mv | AT fawziyahhussain carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT pennyemorton carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT marjoleinsnippe carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT janissullivan carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT charlottefarmer carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT marisalmartinfernandez carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT maddyparsons carmodulatesecadherindynamicsinthepresenceofadenovirustype5 AT georgesantis carmodulatesecadherindynamicsinthepresenceofadenovirustype5 |