Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer

Regulatory T (Treg) cells are potent suppressors that maintain immune homeostasis. Accumulation of Treg can inhibit effective immune responses in cancer patients, leading to tumor development and progression. Despite direct cytotoxicity, several chemotherapeutic drugs have been reported to deplete T...

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Main Authors: Jie-Yao Li, Xiu-Fang Duan, Li-Ping Wang, Yu-Jie Xu, Lan Huang, Teng-Fei Zhang, Jin-Yan Liu, Feng Li, Zhen Zhang, Dong-Li Yue, Fei Wang, Bin Zhang, Yi Zhang
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2014/286170
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author Jie-Yao Li
Xiu-Fang Duan
Li-Ping Wang
Yu-Jie Xu
Lan Huang
Teng-Fei Zhang
Jin-Yan Liu
Feng Li
Zhen Zhang
Dong-Li Yue
Fei Wang
Bin Zhang
Yi Zhang
author_facet Jie-Yao Li
Xiu-Fang Duan
Li-Ping Wang
Yu-Jie Xu
Lan Huang
Teng-Fei Zhang
Jin-Yan Liu
Feng Li
Zhen Zhang
Dong-Li Yue
Fei Wang
Bin Zhang
Yi Zhang
author_sort Jie-Yao Li
collection DOAJ
description Regulatory T (Treg) cells are potent suppressors that maintain immune homeostasis. Accumulation of Treg can inhibit effective immune responses in cancer patients, leading to tumor development and progression. Despite direct cytotoxicity, several chemotherapeutic drugs have been reported to deplete Treg cells for better prognosis for cancer patients. Treg cells are a heterogenous population with at least three different subsets, nonsuppressive, resting, and activated Treg cells. However, the characteristics of Treg cell subsets in lung cancer patients and how chemotherapy affects Treg cells remain elusive. In this study, we first analyzed Treg cell subsets in peripheral blood samples from 40 nonsmall cell lung cancer (NSCLC) patients and 20 healthy donors. Treg cells, specifically activated Treg cell subset, significantly increased in patients with NSCLC. Compared to nonsuppressive Treg cells, activated Treg cells expressed higher level of CD39 and predominantly produced inhibitory cytokines. In vitro assay showed that docetaxel reduced all three subsets of Treg cells. More importantly, we found docetaxel-based chemotherapy significantly decreased all three Treg subsets after 4 cycles of treatment in 17 NSCLC patients. Taken together, this study revealed dynamic changes of various Treg cell subsets in NSCLC patients before and after chemotherapy, providing activated Treg cells as a potential target for chemotherapy.
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spelling doaj-art-87fb1ca87f9943e8954a92330854e2702025-08-20T03:38:05ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/286170286170Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung CancerJie-Yao Li0Xiu-Fang Duan1Li-Ping Wang2Yu-Jie Xu3Lan Huang4Teng-Fei Zhang5Jin-Yan Liu6Feng Li7Zhen Zhang8Dong-Li Yue9Fei Wang10Bin Zhang11Yi Zhang12Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaBiotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaBiotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaBiotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaBiotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Medicine-Division of Hematology/Oncology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USADepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaRegulatory T (Treg) cells are potent suppressors that maintain immune homeostasis. Accumulation of Treg can inhibit effective immune responses in cancer patients, leading to tumor development and progression. Despite direct cytotoxicity, several chemotherapeutic drugs have been reported to deplete Treg cells for better prognosis for cancer patients. Treg cells are a heterogenous population with at least three different subsets, nonsuppressive, resting, and activated Treg cells. However, the characteristics of Treg cell subsets in lung cancer patients and how chemotherapy affects Treg cells remain elusive. In this study, we first analyzed Treg cell subsets in peripheral blood samples from 40 nonsmall cell lung cancer (NSCLC) patients and 20 healthy donors. Treg cells, specifically activated Treg cell subset, significantly increased in patients with NSCLC. Compared to nonsuppressive Treg cells, activated Treg cells expressed higher level of CD39 and predominantly produced inhibitory cytokines. In vitro assay showed that docetaxel reduced all three subsets of Treg cells. More importantly, we found docetaxel-based chemotherapy significantly decreased all three Treg subsets after 4 cycles of treatment in 17 NSCLC patients. Taken together, this study revealed dynamic changes of various Treg cell subsets in NSCLC patients before and after chemotherapy, providing activated Treg cells as a potential target for chemotherapy.http://dx.doi.org/10.1155/2014/286170
spellingShingle Jie-Yao Li
Xiu-Fang Duan
Li-Ping Wang
Yu-Jie Xu
Lan Huang
Teng-Fei Zhang
Jin-Yan Liu
Feng Li
Zhen Zhang
Dong-Li Yue
Fei Wang
Bin Zhang
Yi Zhang
Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
Journal of Immunology Research
title Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
title_full Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
title_fullStr Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
title_full_unstemmed Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
title_short Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer
title_sort selective depletion of regulatory t cell subsets by docetaxel treatment in patients with nonsmall cell lung cancer
url http://dx.doi.org/10.1155/2014/286170
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