Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells
Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
1997-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97221st01 |
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| _version_ | 1850152225803862016 |
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| author | Michael C. Eaton Jennifer E. Hardingham Dusan Kotasek Alexander Dobrovic |
| author_facet | Michael C. Eaton Jennifer E. Hardingham Dusan Kotasek Alexander Dobrovic |
| author_sort | Michael C. Eaton |
| collection | DOAJ |
| description | Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 106 leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment. |
| format | Article |
| id | doaj-art-87f246cfdb674851954187f451f2c2c6 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-87f246cfdb674851954187f451f2c2c62025-08-20T02:26:02ZengTaylor & Francis GroupBioTechniques0736-62051940-98181997-01-0122110010510.2144/97221st01Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor CellsMichael C. Eaton0Jennifer E. Hardingham1Dusan Kotasek2Alexander Dobrovic31University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, Australia1University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, Australia1University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, Australia1University of Adelaide, The Queen Elizabeth Hospital, Woodville, SA, AustraliaDetection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 106 leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment.https://www.future-science.com/doi/10.2144/97221st01 |
| spellingShingle | Michael C. Eaton Jennifer E. Hardingham Dusan Kotasek Alexander Dobrovic Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells BioTechniques |
| title | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
| title_full | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
| title_fullStr | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
| title_full_unstemmed | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
| title_short | Immunobead RT-PCR: A Sensitive Method for Detection of Circulating Tumor Cells |
| title_sort | immunobead rt pcr a sensitive method for detection of circulating tumor cells |
| url | https://www.future-science.com/doi/10.2144/97221st01 |
| work_keys_str_mv | AT michaelceaton immunobeadrtpcrasensitivemethodfordetectionofcirculatingtumorcells AT jenniferehardingham immunobeadrtpcrasensitivemethodfordetectionofcirculatingtumorcells AT dusankotasek immunobeadrtpcrasensitivemethodfordetectionofcirculatingtumorcells AT alexanderdobrovic immunobeadrtpcrasensitivemethodfordetectionofcirculatingtumorcells |