Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes
The genus Haemonchus is the major abomasal parasite of ruminants responsible for substantial economic losses in tropical and temperate regions. This study was conducted to clarify the morphometry and molecular characterisation of Haemonchus species isolated from sheep in Babati district, Tanzania. A...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2023/1923804 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849402362903396352 |
|---|---|
| author | Barakaeli Abdieli Ndosi Dongmin Lee Mohammed Mebarek Bia Heejae Yang Min-Ji Hong Sungbo Seo Hansol Park Keeseon S. Eom |
| author_facet | Barakaeli Abdieli Ndosi Dongmin Lee Mohammed Mebarek Bia Heejae Yang Min-Ji Hong Sungbo Seo Hansol Park Keeseon S. Eom |
| author_sort | Barakaeli Abdieli Ndosi |
| collection | DOAJ |
| description | The genus Haemonchus is the major abomasal parasite of ruminants responsible for substantial economic losses in tropical and temperate regions. This study was conducted to clarify the morphometry and molecular characterisation of Haemonchus species isolated from sheep in Babati district, Tanzania. A total of 486 trichostrongylid nematodes were recovered from five sheep. Of the total worms, 106 nematodes were distinguished by 37 males and 69 females. The asymmetrical length of dorsal ray and the distance of bulb at the apex of spicules were used for identification of males. In females, the linguiform vulvar flap was the most predominant with 33 out of 69 (48%) compared with knobbed morph type which was 25/69 (36%) and smooth morph type with 11/69 (16%). Partial cox1 sequence fragments of Haemonchus contortus isolates showed 98.8%, 99.3%, 99.7%, 99.5%, 99.3%, and 98.4% in male, smooth, knobbed, linguiform A, linguiform B, and linguiform C, respectively; with the average nucleotide divergence ranged from 1.03 to 2.35%. The amplified fragments of ITS-2 genes in knobbed, linguiform A, and smooth morphotypes revealed 99.4%, 98.5%, and 98.3%, respectively. Phylogenetic analysis was evaluated by employing Bayesian inference and maximum-likelihood, and the tree was distinctly separated into three clusters focusing on H. contortus in cluster I within the family Haemonchidae. Genetic drifting, mutation, and modification of the morphological features of the Haemonchus species described to have an impact on the development of drug resistance. Species identification is necessary to understand which species infect animal host. We recommend more studies on the parasites intensity and the strategies for controlling Haemonchus species in Tanzania. |
| format | Article |
| id | doaj-art-87f234cba2ab41cabf513060118f7f24 |
| institution | Kabale University |
| issn | 2090-0031 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-87f234cba2ab41cabf513060118f7f242025-08-20T03:37:33ZengWileyJournal of Parasitology Research2090-00312023-01-01202310.1155/2023/1923804Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genesBarakaeli Abdieli Ndosi0Dongmin Lee1Mohammed Mebarek Bia2Heejae Yang3Min-Ji Hong4Sungbo Seo5Hansol Park6Keeseon S. Eom7Department of ParasitologyDepartment of ParasitologyDepartment of ParasitologyCocoon Inc. #704Cocoon Inc. #704Cocoon Inc. #704Department of ParasitologyDepartment of ParasitologyThe genus Haemonchus is the major abomasal parasite of ruminants responsible for substantial economic losses in tropical and temperate regions. This study was conducted to clarify the morphometry and molecular characterisation of Haemonchus species isolated from sheep in Babati district, Tanzania. A total of 486 trichostrongylid nematodes were recovered from five sheep. Of the total worms, 106 nematodes were distinguished by 37 males and 69 females. The asymmetrical length of dorsal ray and the distance of bulb at the apex of spicules were used for identification of males. In females, the linguiform vulvar flap was the most predominant with 33 out of 69 (48%) compared with knobbed morph type which was 25/69 (36%) and smooth morph type with 11/69 (16%). Partial cox1 sequence fragments of Haemonchus contortus isolates showed 98.8%, 99.3%, 99.7%, 99.5%, 99.3%, and 98.4% in male, smooth, knobbed, linguiform A, linguiform B, and linguiform C, respectively; with the average nucleotide divergence ranged from 1.03 to 2.35%. The amplified fragments of ITS-2 genes in knobbed, linguiform A, and smooth morphotypes revealed 99.4%, 98.5%, and 98.3%, respectively. Phylogenetic analysis was evaluated by employing Bayesian inference and maximum-likelihood, and the tree was distinctly separated into three clusters focusing on H. contortus in cluster I within the family Haemonchidae. Genetic drifting, mutation, and modification of the morphological features of the Haemonchus species described to have an impact on the development of drug resistance. Species identification is necessary to understand which species infect animal host. We recommend more studies on the parasites intensity and the strategies for controlling Haemonchus species in Tanzania.http://dx.doi.org/10.1155/2023/1923804 |
| spellingShingle | Barakaeli Abdieli Ndosi Dongmin Lee Mohammed Mebarek Bia Heejae Yang Min-Ji Hong Sungbo Seo Hansol Park Keeseon S. Eom Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes Journal of Parasitology Research |
| title | Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes |
| title_full | Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes |
| title_fullStr | Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes |
| title_full_unstemmed | Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes |
| title_short | Morphometry and Molecular Identification of Haemonchus Cobb, 1898 (Trichostrongylidae: Nematoda) Isolates from Small Ruminants in Tanzania Based on Mitochondrial cox 1 and rRNA-ITS genes |
| title_sort | morphometry and molecular identification of haemonchus cobb 1898 trichostrongylidae nematoda isolates from small ruminants in tanzania based on mitochondrial cox 1 and rrna its genes |
| url | http://dx.doi.org/10.1155/2023/1923804 |
| work_keys_str_mv | AT barakaeliabdielindosi morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT dongminlee morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT mohammedmebarekbia morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT heejaeyang morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT minjihong morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT sungboseo morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT hansolpark morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes AT keeseonseom morphometryandmolecularidentificationofhaemonchuscobb1898trichostrongylidaenematodaisolatesfromsmallruminantsintanzaniabasedonmitochondrialcox1andrrnaitsgenes |