Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt
Introduction: This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants...
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The Journal of Infection in Developing Countries
2021-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12668 |
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| author | Khaled Abd EL-Hamid Abd El-Razik Amany A Arafa Ehab A Fouad Abdelgayed M Younes Abdulaziz M Almuzaini Amr M Abdou |
| author_facet | Khaled Abd EL-Hamid Abd El-Razik Amany A Arafa Ehab A Fouad Abdelgayed M Younes Abdulaziz M Almuzaini Amr M Abdou |
| author_sort | Khaled Abd EL-Hamid Abd El-Razik |
| collection | DOAJ |
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Introduction: This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to assess the genetic variation in isolated strains of S. agalactiae.
Methodology: Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR.
Results: A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively.
Conclusions: The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt.
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| format | Article |
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| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-87f0470ee3bf475282da83d5cf42ba532025-08-20T02:57:52ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-08-01150810.3855/jidc.12668Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in EgyptKhaled Abd EL-Hamid Abd El-Razik0Amany A Arafa1Ehab A Fouad2Abdelgayed M Younes3Abdulaziz M Almuzaini4Amr M Abdou5Department of Animal Reproduction, National Research Center, Giza, EgyptDepartment of Microbiology and Immunology, National Research Center, Giza, EgyptDepartment of Microbiology and Immunology, National Research Center, Giza, EgyptDepartment of Hydrobiology, National Research Center, Giza, EgyptDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi ArabiaDepartment of Microbiology and Immunology, National Research Center, Giza, Egypt Introduction: This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to assess the genetic variation in isolated strains of S. agalactiae. Methodology: Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR. Results: A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively. Conclusions: The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. https://jidc.org/index.php/journal/article/view/12668S.agalctiaeSubclinical mastitisVirulence genesPCR |
| spellingShingle | Khaled Abd EL-Hamid Abd El-Razik Amany A Arafa Ehab A Fouad Abdelgayed M Younes Abdulaziz M Almuzaini Amr M Abdou Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt Journal of Infection in Developing Countries S.agalctiae Subclinical mastitis Virulence genes PCR |
| title | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
| title_full | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
| title_fullStr | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
| title_full_unstemmed | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
| title_short | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
| title_sort | isolation identification and virulence determinants of streptococcus agalactiae from bovine subclinical mastitis in egypt |
| topic | S.agalctiae Subclinical mastitis Virulence genes PCR |
| url | https://jidc.org/index.php/journal/article/view/12668 |
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