Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes
Introduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of a...
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The Journal of Infection in Developing Countries
2014-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3978 |
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| author | Diana Carolina Quintero-Gil Marta Ospina Jorge Emilio Osorio-Benitez Marlen Martinez-Gutierrez |
| author_facet | Diana Carolina Quintero-Gil Marta Ospina Jorge Emilio Osorio-Benitez Marlen Martinez-Gutierrez |
| author_sort | Diana Carolina Quintero-Gil |
| collection | DOAJ |
| description | Introduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of all four DENV serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the DENV-2 and DENV-3 strains under individual infection and coinfection conditions.
Methodology: C6/36HT cells were infected with the two strains individually or simultaneously (coinfection). Replication capacity was evaluated by RT-qPCR, and the effects on cell viability were assessed with an MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Additionally, Aedes aegypti mosquitoes were artificially fed the two strains of each serotype individually or simultaneously. The viral genomes were quantified by RT-qPCR and the survival of the infected mosquitoes was compared to that of uninfected controls.
Results: In single infections, three strains significantly affected C6/36HT cell viability, but no significant differences were found in the replication capacities of the strains of the same serotype. In the in vivo infections, mosquito survival was not affected, and no significant differences in replication between strains of the same serotype were found. Finally, in coinfections, serotype 2 replicated with a thousandfold greater efficiency than serotype 3 did both in vitro and in vivo.
Conclusions: Due to the cocirculation of serotypes in endemic regions, further studies of coinfections in a natural environment would further an understanding of the transmission dynamics that affect DENV infection epidemiology.
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| format | Article |
| id | doaj-art-87f031411f2e4b55b17c9acbd58269d3 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-87f031411f2e4b55b17c9acbd58269d32025-08-20T02:16:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-07-0180710.3855/jidc.3978Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoesDiana Carolina Quintero-Gil0Marta Ospina1Jorge Emilio Osorio-Benitez2Marlen Martinez-Gutierrez3Programa de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Medellín, ColombiaSecretaria Seccional de Salud y Protección Social de Antioquia, Medellín, ColombiaSchool of Veterinary Medicine, University of Wisconsin, Madison, WI, United StatesPrograma de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Medellín, ColombiaIntroduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of all four DENV serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the DENV-2 and DENV-3 strains under individual infection and coinfection conditions. Methodology: C6/36HT cells were infected with the two strains individually or simultaneously (coinfection). Replication capacity was evaluated by RT-qPCR, and the effects on cell viability were assessed with an MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Additionally, Aedes aegypti mosquitoes were artificially fed the two strains of each serotype individually or simultaneously. The viral genomes were quantified by RT-qPCR and the survival of the infected mosquitoes was compared to that of uninfected controls. Results: In single infections, three strains significantly affected C6/36HT cell viability, but no significant differences were found in the replication capacities of the strains of the same serotype. In the in vivo infections, mosquito survival was not affected, and no significant differences in replication between strains of the same serotype were found. Finally, in coinfections, serotype 2 replicated with a thousandfold greater efficiency than serotype 3 did both in vitro and in vivo. Conclusions: Due to the cocirculation of serotypes in endemic regions, further studies of coinfections in a natural environment would further an understanding of the transmission dynamics that affect DENV infection epidemiology. https://jidc.org/index.php/journal/article/view/3978dengue virusreplication capacityAedes aegyptiC6/36 cells |
| spellingShingle | Diana Carolina Quintero-Gil Marta Ospina Jorge Emilio Osorio-Benitez Marlen Martinez-Gutierrez Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes Journal of Infection in Developing Countries dengue virus replication capacity Aedes aegypti C6/36 cells |
| title | Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes |
| title_full | Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes |
| title_fullStr | Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes |
| title_full_unstemmed | Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes |
| title_short | Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes |
| title_sort | differential replication of dengue virus serotypes 2 and 3 in coinfections of c6 36 cells and aedes aegypti mosquitoes |
| topic | dengue virus replication capacity Aedes aegypti C6/36 cells |
| url | https://jidc.org/index.php/journal/article/view/3978 |
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