Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension
In the broiler industry, intensive genetic selection has been placed on muscle growth which has undesirably led to increased fat accretion. Models of chicken preadipocyte differentiation in vitro have conventionally used incubators without the ability to control oxygen (O2) tension; thus, the cells...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912401109X |
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| author | Jéssica Moraes Cruvinel Elizabeth S. Greene Rachel W. Read Kichoon Lee Sami Dridi Paula R. Chen |
| author_facet | Jéssica Moraes Cruvinel Elizabeth S. Greene Rachel W. Read Kichoon Lee Sami Dridi Paula R. Chen |
| author_sort | Jéssica Moraes Cruvinel |
| collection | DOAJ |
| description | In the broiler industry, intensive genetic selection has been placed on muscle growth which has undesirably led to increased fat accretion. Models of chicken preadipocyte differentiation in vitro have conventionally used incubators without the ability to control oxygen (O2) tension; thus, the cells are exposed to atmospheric (∼20-21%) O2, which is supraphysiological compared to the O2 tension within adipose tissue. The objective of this study was to investigate embryonic broiler preadipocyte differentiation at different O2 tensions, including atmospheric (20%), physiological (5%), and hypoxic (1%). Culture at 1% O2 resulted in increased abundance of HIF1α, a canonical protein stabilized during hypoxia, thus confirming effectiveness of the treatment. Increased accumulation of lipid was observed in preadipocytes cultured in adipogenic differentiation medium compared to the control medium. When considering oxygen tension, lipid accumulation was increased in preadipocytes that were cultured in differentiation medium at 20% O2 compared to 5% or 1% O2. Furthermore, abundance of transcripts related to fatty acid transport and adipogenesis, fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma (PPARγ), were increased in differentiated preadipocytes cultured at 20% O2 compared to 5% or 1% O2. Abundance of transcripts related to lipid synthesis and oxidation, acyl-CoA synthetase long chain family member 1 (ACSL1) and carnitine palmitoyltransferase 1A (CPT1A), were increased in the differentiation cultures compared to the control cultures. Abundance of glutathione peroxidase 4 (GPX4) was increased in all the differentiation cultures compared to the controls, regardless of oxygen tension; however, differences in the abundance of other antioxidant enzymes were not observed. Overall, exposure to atmospheric oxygen tension promotes lipid accumulation within chicken preadipocytes, which may need to be considered when developing in vitro models of this process. |
| format | Article |
| id | doaj-art-87eed9eab8a34473a8629485f3bca890 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-87eed9eab8a34473a8629485f3bca8902025-01-22T05:40:22ZengElsevierPoultry Science0032-57912025-01-011041104531Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tensionJéssica Moraes Cruvinel0Elizabeth S. Greene1Rachel W. Read2Kichoon Lee3Sami Dridi4Paula R. Chen5Division of Animal Sciences, University of Missouri, Columbia 65211, USAUniversity of Arkansas, Center of Excellence for Poultry Science, Fayetteville, AR 72701, USADivision of Animal Sciences, University of Missouri, Columbia 65211, USADepartment of Animal Sciences, The Ohio State University, Columbus, OH 43210, USAUniversity of Arkansas, Center of Excellence for Poultry Science, Fayetteville, AR 72701, USAUSDA-ARS, Plant Genetics Research Unit, Columbia, MO 65211, USA; Corresponding author.In the broiler industry, intensive genetic selection has been placed on muscle growth which has undesirably led to increased fat accretion. Models of chicken preadipocyte differentiation in vitro have conventionally used incubators without the ability to control oxygen (O2) tension; thus, the cells are exposed to atmospheric (∼20-21%) O2, which is supraphysiological compared to the O2 tension within adipose tissue. The objective of this study was to investigate embryonic broiler preadipocyte differentiation at different O2 tensions, including atmospheric (20%), physiological (5%), and hypoxic (1%). Culture at 1% O2 resulted in increased abundance of HIF1α, a canonical protein stabilized during hypoxia, thus confirming effectiveness of the treatment. Increased accumulation of lipid was observed in preadipocytes cultured in adipogenic differentiation medium compared to the control medium. When considering oxygen tension, lipid accumulation was increased in preadipocytes that were cultured in differentiation medium at 20% O2 compared to 5% or 1% O2. Furthermore, abundance of transcripts related to fatty acid transport and adipogenesis, fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma (PPARγ), were increased in differentiated preadipocytes cultured at 20% O2 compared to 5% or 1% O2. Abundance of transcripts related to lipid synthesis and oxidation, acyl-CoA synthetase long chain family member 1 (ACSL1) and carnitine palmitoyltransferase 1A (CPT1A), were increased in the differentiation cultures compared to the control cultures. Abundance of glutathione peroxidase 4 (GPX4) was increased in all the differentiation cultures compared to the controls, regardless of oxygen tension; however, differences in the abundance of other antioxidant enzymes were not observed. Overall, exposure to atmospheric oxygen tension promotes lipid accumulation within chicken preadipocytes, which may need to be considered when developing in vitro models of this process.http://www.sciencedirect.com/science/article/pii/S003257912401109XAdipogenesisLipidsBroilerOxygen tensionPreadipocyte |
| spellingShingle | Jéssica Moraes Cruvinel Elizabeth S. Greene Rachel W. Read Kichoon Lee Sami Dridi Paula R. Chen Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension Poultry Science Adipogenesis Lipids Broiler Oxygen tension Preadipocyte |
| title | Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| title_full | Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| title_fullStr | Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| title_full_unstemmed | Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| title_short | Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| title_sort | research note increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension |
| topic | Adipogenesis Lipids Broiler Oxygen tension Preadipocyte |
| url | http://www.sciencedirect.com/science/article/pii/S003257912401109X |
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