Studies on the immunization of bacterin against bacterial diseases of cultivated soft-shelled turtle

The vaccination tests and field prevention trials against bacterial diseases of soft-shelled turtle (Trionyx sinensis) were carried out in several turtle farms in Huzhou, Xiaoshan etc. from 1997-1998. Aeromonas sobria, isolated mainly from bacterial diseases of cultivated soft-shelled turtle, were i...

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Bibliographic Details
Main Authors: SHEN Jin-yu, YIN Wen-lin, QIAN Dong, CAO Zheng, SHEN Zhi-hua
Format: Article
Language:English
Published: Zhejiang University Press 2000-05-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2000.03.0325
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Summary:The vaccination tests and field prevention trials against bacterial diseases of soft-shelled turtle (Trionyx sinensis) were carried out in several turtle farms in Huzhou, Xiaoshan etc. from 1997-1998. Aeromonas sobria, isolated mainly from bacterial diseases of cultivated soft-shelled turtle, were inactivated with 3.5% formalin for 24 hours. An Al (OH) 3 adjuvant vaccine, with concentration 6×10<sup>9</sup>cfu/mL, was prepared with four-strain inactivated bacteria. The agglutinating titers in serum of immunized mice and turtle could be as high as 2<sup>9</sup> and 2<sup>7</sup>, respectively, on 21-35 days and 15 days after inoculation. The relative survival percent (RSP, %) of both mice and turtle were 100% on the 30th days after vaccination. In field test, the mortality of immunized turtles was decreased by 50% compared with unimmunized group. The results showed this vaccine had a good protection and a practical prospect.
ISSN:1008-9209
2097-5155