Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier
Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this st...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000228 |
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| author | Yang Liu Qing Liu Chengcheng Zhang Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai |
| author_facet | Yang Liu Qing Liu Chengcheng Zhang Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes (such as COG0438, COG0463, and COG2244) related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction. |
| format | Article |
| id | doaj-art-87c92105557c4964883c09788ad849c0 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-87c92105557c4964883c09788ad849c02025-02-03T06:52:50ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112515261537Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrierYang Liu0Qing Liu1Chengcheng Zhang2Jianxin Zhao3Hao Zhang4Wei Chen5Qixiao Zhai6State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China; Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China; Correspondence to: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes (such as COG0438, COG0463, and COG2244) related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction.http://www.sciencedirect.com/science/article/pii/S2213453023000228Akkermansia muciniphilaIntestinal barrierStrain specificityComparative genomicsBacterial surface components |
| spellingShingle | Yang Liu Qing Liu Chengcheng Zhang Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier Food Science and Human Wellness Akkermansia muciniphila Intestinal barrier Strain specificity Comparative genomics Bacterial surface components |
| title | Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier |
| title_full | Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier |
| title_fullStr | Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier |
| title_full_unstemmed | Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier |
| title_short | Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier |
| title_sort | strain specific effects of akkermansia muciniphila on the regulation of intestinal barrier |
| topic | Akkermansia muciniphila Intestinal barrier Strain specificity Comparative genomics Bacterial surface components |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000228 |
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