Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration
To clarify the key material basis for the rapid softening of shrimp muscle during refrigeration, this study established a specific histological detection method for cytoskeletal proteins and analyzed the correlation between the degradation of cytoskeletal proteins and texture deterioration. By compa...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2025-02-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-4-028.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850189999572516864 |
|---|---|
| author | CAI Peifeng, LIAO Weilong, FENG Jun, HU Li, LI Caihong, XU Defeng |
| author_facet | CAI Peifeng, LIAO Weilong, FENG Jun, HU Li, LI Caihong, XU Defeng |
| author_sort | CAI Peifeng, LIAO Weilong, FENG Jun, HU Li, LI Caihong, XU Defeng |
| collection | DOAJ |
| description | To clarify the key material basis for the rapid softening of shrimp muscle during refrigeration, this study established a specific histological detection method for cytoskeletal proteins and analyzed the correlation between the degradation of cytoskeletal proteins and texture deterioration. By comparing the effects of different fixatives, dehydration modes, clarification times, and staining parameters on the staining of cytoskeletal proteins, it was found that the suitable fixative solution for shrimp muscle tissue was Carnoy’s fixative; the specific staining process encompassed five steps: 1) 15 h fixation at room temperature, 2) dehydration with a series of gradient ethanol concentrations, 3) sequential clarification with a mixture of xylene and ethanol followed by xylene, 4) paraffin embedding for sectioning, 5) and Masson’s trichrome staining, clearly showing the morphology of nuclei, myofibrils, and collagen fibers. The pathological results showed that the gap between muscle fiber bundles increased with refrigeration time, and the structure of myofibrillar bundles was partially destroyed. The color of stained collagen fibers became gradually lighter indicating a decrease in its amount. Quantitative analysis showed that refrigeration led to a significant decrease in the volume fraction of collagen fibers and myofibrils in muscle tissue (P < 0.05). The volume fraction of myofibrils in cross-section and longitudinal sections decreased from (91.03 ± 1.37)% and (93.39 ± 0.91)% to (41.14 ± 3.78)% and (24.36 ± 3.67)%, respectively, over a 5-day refrigeration period. The cross-sectional and longitudinal areas of shrimp myofibrils and collagen fibers were significantly positively correlated with muscle hardness and elasticity (P < 0.05), indicating that the reduction in myofibril and collagen contents during refrigeration was closely related to texture deterioration. The significant correlation between the structural degradation of cytoskeletal proteins and texture deterioration revealed their key role in texture preservation and was an important target for early monitoring and intervention of muscle softening. |
| format | Article |
| id | doaj-art-87bb1e261c774e228aaaf6e1ac17eff0 |
| institution | OA Journals |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-87bb1e261c774e228aaaf6e1ac17eff02025-08-20T02:15:25ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-0146425225910.7506/spkx1002-6630-20240823-172Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during RefrigerationCAI Peifeng, LIAO Weilong, FENG Jun, HU Li, LI Caihong, XU Defeng0(1. School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524000, China; 2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 3. School of Medical Technology, Guangdong Medical University, Zhanjiang 524000, China; 4. School of Basic Medicine, Guangdong Medical University, Zhanjiang 524000, China)To clarify the key material basis for the rapid softening of shrimp muscle during refrigeration, this study established a specific histological detection method for cytoskeletal proteins and analyzed the correlation between the degradation of cytoskeletal proteins and texture deterioration. By comparing the effects of different fixatives, dehydration modes, clarification times, and staining parameters on the staining of cytoskeletal proteins, it was found that the suitable fixative solution for shrimp muscle tissue was Carnoy’s fixative; the specific staining process encompassed five steps: 1) 15 h fixation at room temperature, 2) dehydration with a series of gradient ethanol concentrations, 3) sequential clarification with a mixture of xylene and ethanol followed by xylene, 4) paraffin embedding for sectioning, 5) and Masson’s trichrome staining, clearly showing the morphology of nuclei, myofibrils, and collagen fibers. The pathological results showed that the gap between muscle fiber bundles increased with refrigeration time, and the structure of myofibrillar bundles was partially destroyed. The color of stained collagen fibers became gradually lighter indicating a decrease in its amount. Quantitative analysis showed that refrigeration led to a significant decrease in the volume fraction of collagen fibers and myofibrils in muscle tissue (P < 0.05). The volume fraction of myofibrils in cross-section and longitudinal sections decreased from (91.03 ± 1.37)% and (93.39 ± 0.91)% to (41.14 ± 3.78)% and (24.36 ± 3.67)%, respectively, over a 5-day refrigeration period. The cross-sectional and longitudinal areas of shrimp myofibrils and collagen fibers were significantly positively correlated with muscle hardness and elasticity (P < 0.05), indicating that the reduction in myofibril and collagen contents during refrigeration was closely related to texture deterioration. The significant correlation between the structural degradation of cytoskeletal proteins and texture deterioration revealed their key role in texture preservation and was an important target for early monitoring and intervention of muscle softening.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-4-028.pdflitopenaeus vannamei; refrigeration; muscle softening; cytoskeletal proteins; masson’s trichrome staining |
| spellingShingle | CAI Peifeng, LIAO Weilong, FENG Jun, HU Li, LI Caihong, XU Defeng Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration Shipin Kexue litopenaeus vannamei; refrigeration; muscle softening; cytoskeletal proteins; masson’s trichrome staining |
| title | Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration |
| title_full | Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration |
| title_fullStr | Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration |
| title_full_unstemmed | Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration |
| title_short | Specific Staining of Muscle Cytoskeletal Proteins in Litopenaeus vannamei and Their Role in Textural Deterioration during Refrigeration |
| title_sort | specific staining of muscle cytoskeletal proteins in litopenaeus vannamei and their role in textural deterioration during refrigeration |
| topic | litopenaeus vannamei; refrigeration; muscle softening; cytoskeletal proteins; masson’s trichrome staining |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-4-028.pdf |
| work_keys_str_mv | AT caipeifengliaoweilongfengjunhulilicaihongxudefeng specificstainingofmusclecytoskeletalproteinsinlitopenaeusvannameiandtheirroleintexturaldeteriorationduringrefrigeration |