Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage
Chlorpyrifos (CPF) is a broad-spectrum organophosphate chloride (OP) insecticide widely used in domestic use and open field. However, there is currently no relevant research revealing the toxic effects of CPF exposure on the skin, then it is necessary to comprehensively understand the toxic effects...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016634 |
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| author | Weibin Du Wenxiang Zeng Zhenwei Wang Huahui Hu Hongfeng Ruan Gang Qu |
| author_facet | Weibin Du Wenxiang Zeng Zhenwei Wang Huahui Hu Hongfeng Ruan Gang Qu |
| author_sort | Weibin Du |
| collection | DOAJ |
| description | Chlorpyrifos (CPF) is a broad-spectrum organophosphate chloride (OP) insecticide widely used in domestic use and open field. However, there is currently no relevant research revealing the toxic effects of CPF exposure on the skin, then it is necessary to comprehensively understand the toxic effects and mechanisms. The results showed that the skin tissue structure and function of mice were significantly disrupted after CPF exposure. Furthermore, PPI network analysis and molecular docking experiments pinpointed core targets such as Bcl2, EGFR, Caspase-3, TNF-α, IL-1β, and VEGF. Additionally, through KEGG analysis, the VEGF and apoptosis signaling pathways were identified as pivotal pathways implicated in CPF-induced skin toxicity. These core targets and pathways were subsequently confirmed through animal experimentation. In conclusion, our study suggests that CPF exposure primarily induces skin damage by disrupting tissue structure and function, mediated through apoptosis and proliferation processes in skin cells, alongside aberrant angiogenesis and heightened inflammatory response. These insights are expected to advance the development of preventive and therapeutic strategies aimed at mitigating the adverse effects of CPF exposure on the skin. |
| format | Article |
| id | doaj-art-87b5ad51b0ae49eb87a991297c60fbef |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-87b5ad51b0ae49eb87a991297c60fbef2025-02-12T05:29:54ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117587Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damageWeibin Du0Wenxiang Zeng1Zhenwei Wang2Huahui Hu3Hongfeng Ruan4Gang Qu5Research Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, China; Corresponding authors at: Research Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.Research Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, ChinaResearch Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, ChinaResearch Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, ChinaInstitute of Orthopaedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou, ChinaResearch Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, China; Corresponding authors at: Research Institute of Orthopedics, the Jiangnan Hospital affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.Chlorpyrifos (CPF) is a broad-spectrum organophosphate chloride (OP) insecticide widely used in domestic use and open field. However, there is currently no relevant research revealing the toxic effects of CPF exposure on the skin, then it is necessary to comprehensively understand the toxic effects and mechanisms. The results showed that the skin tissue structure and function of mice were significantly disrupted after CPF exposure. Furthermore, PPI network analysis and molecular docking experiments pinpointed core targets such as Bcl2, EGFR, Caspase-3, TNF-α, IL-1β, and VEGF. Additionally, through KEGG analysis, the VEGF and apoptosis signaling pathways were identified as pivotal pathways implicated in CPF-induced skin toxicity. These core targets and pathways were subsequently confirmed through animal experimentation. In conclusion, our study suggests that CPF exposure primarily induces skin damage by disrupting tissue structure and function, mediated through apoptosis and proliferation processes in skin cells, alongside aberrant angiogenesis and heightened inflammatory response. These insights are expected to advance the development of preventive and therapeutic strategies aimed at mitigating the adverse effects of CPF exposure on the skin.http://www.sciencedirect.com/science/article/pii/S0147651324016634CybertoxicologyChlorpyrifosMolecular dockingApoptosisSkin damage |
| spellingShingle | Weibin Du Wenxiang Zeng Zhenwei Wang Huahui Hu Hongfeng Ruan Gang Qu Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage Ecotoxicology and Environmental Safety Cybertoxicology Chlorpyrifos Molecular docking Apoptosis Skin damage |
| title | Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| title_full | Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| title_fullStr | Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| title_full_unstemmed | Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| title_short | Cybertoxicology, molecular docking, and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| title_sort | cybertoxicology molecular docking and experimental verification reveal the mechanism of action of chlorpyrifos on skin damage |
| topic | Cybertoxicology Chlorpyrifos Molecular docking Apoptosis Skin damage |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016634 |
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