Antibiotic Susceptibility and Resistance Testing: An Overview
The results of in vitro antibiotic susceptibility testing can predict the clinical response to treatment and guide the selection of antibiotics. The minimum inhibitory concentration (MIC) of an organism is the lowest concentration of an antibiotic that will inhibit its growth. Bacteria are classifie...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2000/382415 |
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| _version_ | 1849401685832630272 |
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| author | Fiona Smaill |
| author_facet | Fiona Smaill |
| author_sort | Fiona Smaill |
| collection | DOAJ |
| description | The results of in vitro antibiotic susceptibility testing can predict the clinical response to treatment and guide the selection of antibiotics. The minimum inhibitory concentration (MIC) of an organism is the lowest concentration of an antibiotic that will inhibit its growth. Bacteria are classified as sensitive, intermediate or resistant based on breakpoint MIC values that are arbitrarily defined and reflect the achievable levels of the antibiotic, the distribution of MICs for the organism and their correlation with clinical outcome. Broth dilution, agar dilution and gradient diffusion (the ’E test’), where twofold serial dilutions of antibiotic are incorporated into tubes of broth, agar plates or on a paper strip, respectively, are different methods to measure the MIC of an organism. The disk diffusion method defines an organism as sensitive or resistant based on the extent of its growth around an antibiotic-containing disk. MIC values are influenced by several laboratory factors. To ensure reproducible results, the laboratory must closely follow methods developed by the National Committee for Clinical Laboratory Standards, which defines standard growth media, incubation temperature and environment, the inoculum and quality control parameters. |
| format | Article |
| id | doaj-art-87ad032f91fd483b9c09232c1c917ec2 |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-87ad032f91fd483b9c09232c1c917ec22025-08-20T03:37:43ZengWileyCanadian Journal of Gastroenterology0835-79002000-01-01141087187510.1155/2000/382415Antibiotic Susceptibility and Resistance Testing: An OverviewFiona Smaill0Faculty of Health Sciences, McMaster University, Hamilton, Ontario, CanadaThe results of in vitro antibiotic susceptibility testing can predict the clinical response to treatment and guide the selection of antibiotics. The minimum inhibitory concentration (MIC) of an organism is the lowest concentration of an antibiotic that will inhibit its growth. Bacteria are classified as sensitive, intermediate or resistant based on breakpoint MIC values that are arbitrarily defined and reflect the achievable levels of the antibiotic, the distribution of MICs for the organism and their correlation with clinical outcome. Broth dilution, agar dilution and gradient diffusion (the ’E test’), where twofold serial dilutions of antibiotic are incorporated into tubes of broth, agar plates or on a paper strip, respectively, are different methods to measure the MIC of an organism. The disk diffusion method defines an organism as sensitive or resistant based on the extent of its growth around an antibiotic-containing disk. MIC values are influenced by several laboratory factors. To ensure reproducible results, the laboratory must closely follow methods developed by the National Committee for Clinical Laboratory Standards, which defines standard growth media, incubation temperature and environment, the inoculum and quality control parameters.http://dx.doi.org/10.1155/2000/382415 |
| spellingShingle | Fiona Smaill Antibiotic Susceptibility and Resistance Testing: An Overview Canadian Journal of Gastroenterology |
| title | Antibiotic Susceptibility and Resistance Testing: An Overview |
| title_full | Antibiotic Susceptibility and Resistance Testing: An Overview |
| title_fullStr | Antibiotic Susceptibility and Resistance Testing: An Overview |
| title_full_unstemmed | Antibiotic Susceptibility and Resistance Testing: An Overview |
| title_short | Antibiotic Susceptibility and Resistance Testing: An Overview |
| title_sort | antibiotic susceptibility and resistance testing an overview |
| url | http://dx.doi.org/10.1155/2000/382415 |
| work_keys_str_mv | AT fionasmaill antibioticsusceptibilityandresistancetestinganoverview |