Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>

<i>Lawsonia intracellularis</i> is a Gram-negative, intracellular bacterium that can infect several animal species. In pigs, the bacteria cause porcine proliferative enteropathy, or ileitis. The wide spread of the pathogen produces a large impact on pig production worldwide. Saliva is a...

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Main Authors: Andrea Martínez-Martínez, Manuel Toledo, Emilio Ruiz, Simón García, Anabel Fernández, José Joaquín Cerón, Rut Menjon, María Teresa Tejedor, Elena Goyena, Alberto Muñoz-Prieto
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Language:English
Published: MDPI AG 2025-01-01
Series:Immuno
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Online Access:https://www.mdpi.com/2673-5601/5/1/3
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author Andrea Martínez-Martínez
Manuel Toledo
Emilio Ruiz
Simón García
Anabel Fernández
José Joaquín Cerón
Rut Menjon
María Teresa Tejedor
Elena Goyena
Alberto Muñoz-Prieto
author_facet Andrea Martínez-Martínez
Manuel Toledo
Emilio Ruiz
Simón García
Anabel Fernández
José Joaquín Cerón
Rut Menjon
María Teresa Tejedor
Elena Goyena
Alberto Muñoz-Prieto
author_sort Andrea Martínez-Martínez
collection DOAJ
description <i>Lawsonia intracellularis</i> is a Gram-negative, intracellular bacterium that can infect several animal species. In pigs, the bacteria cause porcine proliferative enteropathy, or ileitis. The wide spread of the pathogen produces a large impact on pig production worldwide. Saliva is a source of biomarkers that can help to monitor changes in the immune system after vaccination. The purpose of this study was to study the changes in haptoglobin (Hp), immunoglobulin G (IgG), and adenosine deaminase (ADA) in saliva after vaccination against <i>Lawsonia intracellularis</i>. In addition, productivity parameters were analysed to evaluate if vaccination and changes in salivary analytes could be associated with changes in these parameters. The pigs vaccinated against Lawsonia showed an improvement in the productive parameters and a reduction in food conversion and frequency of diseases. In addition, they showed lower values of Hp (<i>p</i> = 0.011), IgG (<i>p</i> < 0.01), and ADA (<i>p</i> < 0.003) in saliva during the first two months of the fattening period compared to non-vaccinated pigs. It could be concluded that in our experimental conditions, the vaccination against <i>Lawsonia intracellularis</i> produced a significant decrease in biomarkers of the immune response in saliva compared with the non-vaccinated pigs. This would indicate a reduction in the activation of the immune system, which could be postulated to be due to the increased defence ability of the organism against pathogens. This reduced activation of the immune system can lead to better food conversion and an increase in the productive parameters of these pigs. Overall, this report opens a new window for the possible use of saliva for non-invasive evaluation of the immune system after vaccination in pigs.
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spelling doaj-art-87a33b6fab91417caae14cef85eb09712025-08-20T02:42:31ZengMDPI AGImmuno2673-56012025-01-0151310.3390/immuno5010003Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>Andrea Martínez-Martínez0Manuel Toledo1Emilio Ruiz2Simón García3Anabel Fernández4José Joaquín Cerón5Rut Menjon6María Teresa Tejedor7Elena Goyena8Alberto Muñoz-Prieto9Agropecuaria Casas Nuevas S.A., Ctra. de las Palas, Fuente Álamo, 30320 Murcia, SpainAgropecuaria Casas Nuevas S.A., Ctra. de las Palas, Fuente Álamo, 30320 Murcia, SpainAgropecuaria Casas Nuevas S.A., Ctra. de las Palas, Fuente Álamo, 30320 Murcia, SpainAgropecuaria Casas Nuevas S.A., Ctra. de las Palas, Fuente Álamo, 30320 Murcia, SpainAgropecuaria Casas Nuevas S.A., Ctra. de las Palas, Fuente Álamo, 30320 Murcia, SpainInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, 30100 Murcia, SpainMSD Animal Health España, Carbajosa de la Sagrada, 37188 Salamanca, SpainDepartment of Anatomy, Embryology and Genetics, CIBERCV, Faculty of Veterinary Medicine, University of Zaragoza, 50013 Zaragoza, SpainDepartamento Sanidad Animal, Facultad de Veterinaria, Universidad de Campus de Espinardo s/n, 30100 Murcia, SpainInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain<i>Lawsonia intracellularis</i> is a Gram-negative, intracellular bacterium that can infect several animal species. In pigs, the bacteria cause porcine proliferative enteropathy, or ileitis. The wide spread of the pathogen produces a large impact on pig production worldwide. Saliva is a source of biomarkers that can help to monitor changes in the immune system after vaccination. The purpose of this study was to study the changes in haptoglobin (Hp), immunoglobulin G (IgG), and adenosine deaminase (ADA) in saliva after vaccination against <i>Lawsonia intracellularis</i>. In addition, productivity parameters were analysed to evaluate if vaccination and changes in salivary analytes could be associated with changes in these parameters. The pigs vaccinated against Lawsonia showed an improvement in the productive parameters and a reduction in food conversion and frequency of diseases. In addition, they showed lower values of Hp (<i>p</i> = 0.011), IgG (<i>p</i> < 0.01), and ADA (<i>p</i> < 0.003) in saliva during the first two months of the fattening period compared to non-vaccinated pigs. It could be concluded that in our experimental conditions, the vaccination against <i>Lawsonia intracellularis</i> produced a significant decrease in biomarkers of the immune response in saliva compared with the non-vaccinated pigs. This would indicate a reduction in the activation of the immune system, which could be postulated to be due to the increased defence ability of the organism against pathogens. This reduced activation of the immune system can lead to better food conversion and an increase in the productive parameters of these pigs. Overall, this report opens a new window for the possible use of saliva for non-invasive evaluation of the immune system after vaccination in pigs.https://www.mdpi.com/2673-5601/5/1/3LawsoniasalivavaccineADAhaptoglobinIgG
spellingShingle Andrea Martínez-Martínez
Manuel Toledo
Emilio Ruiz
Simón García
Anabel Fernández
José Joaquín Cerón
Rut Menjon
María Teresa Tejedor
Elena Goyena
Alberto Muñoz-Prieto
Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
Immuno
Lawsonia
saliva
vaccine
ADA
haptoglobin
IgG
title Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
title_full Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
title_fullStr Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
title_full_unstemmed Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
title_short Changes in Analytes Related to Immunity in the Saliva of Pigs After Vaccination Against <i>Lawsonia intracellularis</i>
title_sort changes in analytes related to immunity in the saliva of pigs after vaccination against i lawsonia intracellularis i
topic Lawsonia
saliva
vaccine
ADA
haptoglobin
IgG
url https://www.mdpi.com/2673-5601/5/1/3
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