EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT
Background: One of the problems in Orthopedics which often results in less satisfactory outcomes is the muscle injuries treatment. This is due to slow healing process and outcome has not been optimal because it produces scar tissue. Purpose: To evaluate effect of platelet rich fibrin and allogenic...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2019-12-01
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| Series: | Journal Orthopaedi and Traumatology Surabaya |
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| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/16565 |
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| author | Dwikora Novembri Utomo Mohammad Zaim Chilmi Angga Fiandana |
| author_facet | Dwikora Novembri Utomo Mohammad Zaim Chilmi Angga Fiandana |
| author_sort | Dwikora Novembri Utomo |
| collection | DOAJ |
| description | Background: One of the problems in Orthopedics which often results in less satisfactory outcomes is the muscle injuries treatment. This is due to slow healing process and outcome has not been optimal because it produces scar tissue.
Purpose: To evaluate effect of platelet rich fibrin and allogenic mesenchymal stem cell on muscle regeneration through comparation of myoblast and fibroblast ratio.
Methods: This research is true experimental with randomized pre and post test with control group design. The treatment group included administering allogenic mesenchymal stem cells (MSC), rich fibrin platelets (PRF), and a combination of both. The study subjects were New Zealand White Rabbit which gastrocnemius muscle is incised. Muscle quality evaluation was done in the second and fourth weeks through histology examination in the form of myoblast cell ratio to fibroblast.
Results: In the second week evaluation, administering allogenic MSC combined with PRF gave significant results to an increase in myoblast cell ratio to fibroblast. While evaluation of fourth week, giving of three treatment groups gave significant result to myoblast cell ratio to fibroblast.
Conclusion: Allogenic MSC administration combined with PRF can improve New Zealand White Rabbit muscle healing quality by histological examination. |
| format | Article |
| id | doaj-art-878c8ea6abba4aef978c24ab4497da75 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-878c8ea6abba4aef978c24ab4497da752025-02-12T07:29:23ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422019-12-01729510510.20473/joints.v7i2.2018.95-10513515EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECTDwikora Novembri Utomo0Mohammad Zaim Chilmi1Angga Fiandana2Staff of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga/Dr Soetomo General Hospital, SurabayaStaff of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga/Dr Soetomo General Hospital, SurabayaResident of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga/Dr Soetomo General Hospital, SurabayaBackground: One of the problems in Orthopedics which often results in less satisfactory outcomes is the muscle injuries treatment. This is due to slow healing process and outcome has not been optimal because it produces scar tissue. Purpose: To evaluate effect of platelet rich fibrin and allogenic mesenchymal stem cell on muscle regeneration through comparation of myoblast and fibroblast ratio. Methods: This research is true experimental with randomized pre and post test with control group design. The treatment group included administering allogenic mesenchymal stem cells (MSC), rich fibrin platelets (PRF), and a combination of both. The study subjects were New Zealand White Rabbit which gastrocnemius muscle is incised. Muscle quality evaluation was done in the second and fourth weeks through histology examination in the form of myoblast cell ratio to fibroblast. Results: In the second week evaluation, administering allogenic MSC combined with PRF gave significant results to an increase in myoblast cell ratio to fibroblast. While evaluation of fourth week, giving of three treatment groups gave significant result to myoblast cell ratio to fibroblast. Conclusion: Allogenic MSC administration combined with PRF can improve New Zealand White Rabbit muscle healing quality by histological examination.https://e-journal.unair.ac.id/JOINTS/article/view/16565allogenic mscprfmyoblastfibroblast |
| spellingShingle | Dwikora Novembri Utomo Mohammad Zaim Chilmi Angga Fiandana EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT Journal Orthopaedi and Traumatology Surabaya allogenic msc prf myoblast fibroblast |
| title | EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT |
| title_full | EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT |
| title_fullStr | EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT |
| title_full_unstemmed | EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT |
| title_short | EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT |
| title_sort | effect of platelet rich fibrin and mesenchymal stem cell on myoblast and fibroblasts ratio in rabbit muscle defect |
| topic | allogenic msc prf myoblast fibroblast |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/16565 |
| work_keys_str_mv | AT dwikoranovembriutomo effectofplateletrichfibrinandmesenchymalstemcellonmyoblastandfibroblastsratioinrabbitmuscledefect AT mohammadzaimchilmi effectofplateletrichfibrinandmesenchymalstemcellonmyoblastandfibroblastsratioinrabbitmuscledefect AT anggafiandana effectofplateletrichfibrinandmesenchymalstemcellonmyoblastandfibroblastsratioinrabbitmuscledefect |