Eliminating tissue culture from plant gene editing in the near future: A wish or reality?
Traditional plant breeding methods alone are insufficient to guarantee food security for a growing global population under a changing climate, necessitating more advanced approaches to develop productive and resilient crop varieties. The development of genome editing tools, particularly CRISPR/CAS,...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | Current Plant Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662825000015 |
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| Summary: | Traditional plant breeding methods alone are insufficient to guarantee food security for a growing global population under a changing climate, necessitating more advanced approaches to develop productive and resilient crop varieties. The development of genome editing tools, particularly CRISPR/CAS, are significantly speeding up crop improvement by enabling targeted breeding in most crop species. However, for many crop species, the need for tissue culture remains a major bottle neck, slowing the progress of crop improvement. In this review, we are presenting and discussing approaches for delivering genome editing tools into a wide variety of crop plants, including perennials, and ideally without integration of transgenes. We suggest that efficient non-tissue culture delivery systems for high-performance genome editing are needed to fully reach the genome engineering potential in crop plants. |
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| ISSN: | 2214-6628 |