N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques

African soils are inherently low in mineral nutrients. Incorporating N2-fixing legumes into cropping systems can improve soil fertility and increase crop yields. This study assessed N2 fixation, carbon assimilation, grain mineral accumulation and water-use efficiency of 30 groundnut genotypes grown...

Full description

Saved in:
Bibliographic Details
Main Authors: Titus Y. Ngmenzuma, Richard Oteng-Frimpong, Felix Dapare Dakora
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Agronomy
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fagro.2025.1483741/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850159278203076608
author Titus Y. Ngmenzuma
Richard Oteng-Frimpong
Felix Dapare Dakora
author_facet Titus Y. Ngmenzuma
Richard Oteng-Frimpong
Felix Dapare Dakora
author_sort Titus Y. Ngmenzuma
collection DOAJ
description African soils are inherently low in mineral nutrients. Incorporating N2-fixing legumes into cropping systems can improve soil fertility and increase crop yields. This study assessed N2 fixation, carbon assimilation, grain mineral accumulation and water-use efficiency of 30 groundnut genotypes grown in the field at the Mpumalanga Province, South Africa, using the 15N and 13C natural abundance techniques. The results revealed marked differences in symbiotic performance between and among the groundnut genotypes, with IS-07273 and ICGV13910 exhibiting greater symbiotic dependency on N2 fixation for their N nutrition and higher amounts of N-fixed. The two high N2-fixing symbioses (IS-07273 and ICGV13910) also accumulated significantly high levels of K, Na, Zn, Cu, Mn, and B in their grain. As a result, there were strong correlations between amounts of N-fixed and K, Na, B, Cu, Zn and Mn for genotype ICGV13910. Genotype IS-07273 also showed significant correlations between N-fixed and S, N concentration (%N) and P, %N and K, as well as nodule number and Ca. As to be expected, genotypes with the highest shoot %N accumulated the most protein in their grain. Out of the 30 groundnut genotypes tested in the field, YENYAWOSO, ICGV13848, ICGV13851, ICGV15033 and ICGV131065 showed greater shoot δ13C values, and hence higher water-use efficiency. The high N2 fixation in genotypes ICGV13910 and IS-07273 correlated positively with macro- and micro-nutrient concentrations in their grain, indicating their potential for use in breeding programmes to enhance nutritional security in groundnut.
format Article
id doaj-art-878436225d7b405486df243d2a949047
institution OA Journals
issn 2673-3218
language English
publishDate 2025-06-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Agronomy
spelling doaj-art-878436225d7b405486df243d2a9490472025-08-20T02:23:35ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182025-06-01710.3389/fagro.2025.14837411483741N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniquesTitus Y. Ngmenzuma0Richard Oteng-Frimpong1Felix Dapare Dakora2Department of Crop Sciences, Tshwane University of Technology, Pretoria, South AfricaSavanna Agricultural Research Institute, CSIR, Tamale, GhanaDepartment of Chemistry, Faculty of Science, Tshwane University of Technology, Pretoria, South AfricaAfrican soils are inherently low in mineral nutrients. Incorporating N2-fixing legumes into cropping systems can improve soil fertility and increase crop yields. This study assessed N2 fixation, carbon assimilation, grain mineral accumulation and water-use efficiency of 30 groundnut genotypes grown in the field at the Mpumalanga Province, South Africa, using the 15N and 13C natural abundance techniques. The results revealed marked differences in symbiotic performance between and among the groundnut genotypes, with IS-07273 and ICGV13910 exhibiting greater symbiotic dependency on N2 fixation for their N nutrition and higher amounts of N-fixed. The two high N2-fixing symbioses (IS-07273 and ICGV13910) also accumulated significantly high levels of K, Na, Zn, Cu, Mn, and B in their grain. As a result, there were strong correlations between amounts of N-fixed and K, Na, B, Cu, Zn and Mn for genotype ICGV13910. Genotype IS-07273 also showed significant correlations between N-fixed and S, N concentration (%N) and P, %N and K, as well as nodule number and Ca. As to be expected, genotypes with the highest shoot %N accumulated the most protein in their grain. Out of the 30 groundnut genotypes tested in the field, YENYAWOSO, ICGV13848, ICGV13851, ICGV15033 and ICGV131065 showed greater shoot δ13C values, and hence higher water-use efficiency. The high N2 fixation in genotypes ICGV13910 and IS-07273 correlated positively with macro- and micro-nutrient concentrations in their grain, indicating their potential for use in breeding programmes to enhance nutritional security in groundnut.https://www.frontiersin.org/articles/10.3389/fagro.2025.1483741/fullN2-fixationgrain yieldsoil N uptakeseed protein contentgrain mineral content
spellingShingle Titus Y. Ngmenzuma
Richard Oteng-Frimpong
Felix Dapare Dakora
N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
Frontiers in Agronomy
N2-fixation
grain yield
soil N uptake
seed protein content
grain mineral content
title N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
title_full N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
title_fullStr N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
title_full_unstemmed N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
title_short N2 fixation, grain mineral accumulation, and water-use efficiency in 30 field-grown groundnut (Arachis hypogaea L.) genotypes in Mpumalanga, South Africa, measured using 15N and 13C natural abundance techniques
title_sort n2 fixation grain mineral accumulation and water use efficiency in 30 field grown groundnut arachis hypogaea l genotypes in mpumalanga south africa measured using 15n and 13c natural abundance techniques
topic N2-fixation
grain yield
soil N uptake
seed protein content
grain mineral content
url https://www.frontiersin.org/articles/10.3389/fagro.2025.1483741/full
work_keys_str_mv AT titusyngmenzuma n2fixationgrainmineralaccumulationandwateruseefficiencyin30fieldgrowngroundnutarachishypogaealgenotypesinmpumalangasouthafricameasuredusing15nand13cnaturalabundancetechniques
AT richardotengfrimpong n2fixationgrainmineralaccumulationandwateruseefficiencyin30fieldgrowngroundnutarachishypogaealgenotypesinmpumalangasouthafricameasuredusing15nand13cnaturalabundancetechniques
AT felixdaparedakora n2fixationgrainmineralaccumulationandwateruseefficiencyin30fieldgrowngroundnutarachishypogaealgenotypesinmpumalangasouthafricameasuredusing15nand13cnaturalabundancetechniques