Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode
A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Un...
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China Food Publishing Company
2024-12-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-029.pdf |
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| author | WANG Donghua, WANG Xu, WANG Yuting, GAO Shan, WANG Weining, YANG Fuming, YU Dianyu |
| author_facet | WANG Donghua, WANG Xu, WANG Yuting, GAO Shan, WANG Weining, YANG Fuming, YU Dianyu |
| author_sort | WANG Donghua, WANG Xu, WANG Yuting, GAO Shan, WANG Weining, YANG Fuming, YU Dianyu |
| collection | DOAJ |
| description | A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Under the conditions of ChOx and HRP addition each at 2.5 U and pH 7.5, the sensor showed significant electrochemical response to PC. PC concentrations in the range of 10–300 mg/L were linearly correlated with the peak current, and the detection limit was 2 mg/L. Recoveries for spiked samples ranged from 90% to 110% with relative standard deviations (RSDs) less than 4.68%. Good correlation was observed between the results of the biosensor and those of high performance liquid chromatography (HPLC) with a correlation coefficient (r) of 0.992 9. After 14 days of storage, the sensor still retained 98% of its initial current value, indicating its good stability. This study can provide theoretical support for the rapid inspection and control of vegetable oil refining. |
| format | Article |
| id | doaj-art-8782f1487a5542ba8899a1da231a48dd |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-8782f1487a5542ba8899a1da231a48dd2025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452425926510.7506/spkx1002-6630-20240508-049Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon ElectrodeWANG Donghua, WANG Xu, WANG Yuting, GAO Shan, WANG Weining, YANG Fuming, YU Dianyu0(1. Heilongjiang Green Food Science Research Institute, Harbin 150030, China; 2. Faculty of Engineering, The University of Sheffield, Sheffield S10 2TN, UK; 3. College of Food Science, Northeast Agricultural University, Harbin 150030, China)A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Under the conditions of ChOx and HRP addition each at 2.5 U and pH 7.5, the sensor showed significant electrochemical response to PC. PC concentrations in the range of 10–300 mg/L were linearly correlated with the peak current, and the detection limit was 2 mg/L. Recoveries for spiked samples ranged from 90% to 110% with relative standard deviations (RSDs) less than 4.68%. Good correlation was observed between the results of the biosensor and those of high performance liquid chromatography (HPLC) with a correlation coefficient (r) of 0.992 9. After 14 days of storage, the sensor still retained 98% of its initial current value, indicating its good stability. This study can provide theoretical support for the rapid inspection and control of vegetable oil refining.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-029.pdfglassy carbon electrode; biosensors; artificial crude soybean oil; phosphatidylcholine; quick detection |
| spellingShingle | WANG Donghua, WANG Xu, WANG Yuting, GAO Shan, WANG Weining, YANG Fuming, YU Dianyu Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode Shipin Kexue glassy carbon electrode; biosensors; artificial crude soybean oil; phosphatidylcholine; quick detection |
| title | Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode |
| title_full | Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode |
| title_fullStr | Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode |
| title_full_unstemmed | Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode |
| title_short | Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode |
| title_sort | determination of phosphatidylcholine in artificial crude soybean oil by biosensor based on horseradish peroxidase choline oxidase modified glassy carbon electrode |
| topic | glassy carbon electrode; biosensors; artificial crude soybean oil; phosphatidylcholine; quick detection |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-029.pdf |
| work_keys_str_mv | AT wangdonghuawangxuwangyutinggaoshanwangweiningyangfumingyudianyu determinationofphosphatidylcholineinartificialcrudesoybeanoilbybiosensorbasedonhorseradishperoxidasecholineoxidasemodifiedglassycarbonelectrode |