Determination of Phosphatidylcholine in Artificial Crude Soybean Oil by Biosensor Based on Horseradish Peroxidase/Choline Oxidase-Modified Glassy Carbon Electrode
A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Un...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2024-12-01
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| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-029.pdf |
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| Summary: | A biosensor for the determination of phosphatidylcholine (PC) in artificial crude soybean oil was fabricated by immobilizing horseradish peroxidase (HRP) and choline oxidase (ChOx) onto on a glassy carbon electrode (GCE) modified with multi-walled carbon nanotube (MWCNTs), SnO2 and chitosan (CS). Under the conditions of ChOx and HRP addition each at 2.5 U and pH 7.5, the sensor showed significant electrochemical response to PC. PC concentrations in the range of 10–300 mg/L were linearly correlated with the peak current, and the detection limit was 2 mg/L. Recoveries for spiked samples ranged from 90% to 110% with relative standard deviations (RSDs) less than 4.68%. Good correlation was observed between the results of the biosensor and those of high performance liquid chromatography (HPLC) with a correlation coefficient (r) of 0.992 9. After 14 days of storage, the sensor still retained 98% of its initial current value, indicating its good stability. This study can provide theoretical support for the rapid inspection and control of vegetable oil refining. |
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| ISSN: | 1002-6630 |