Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages
Total RNA was extracted from mammary gland of mice. Two primers were designed and synthesized according to the murine lactoferrin gene reported in Genbank. A cDNA fragment about 897 bp in length was obtained through RT-PCR. The PCR product was cloned into pGEM-T vector and then sequenced. Sequence a...
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| Main Authors: | WANG Yi-zhen, HAN Fei-fei, HUANG Hai-qing |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0083 |
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