Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages
Total RNA was extracted from mammary gland of mice. Two primers were designed and synthesized according to the murine lactoferrin gene reported in Genbank. A cDNA fragment about 897 bp in length was obtained through RT-PCR. The PCR product was cloned into pGEM-T vector and then sequenced. Sequence a...
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Zhejiang University Press
2004-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0083 |
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| author | WANG Yi-zhen HAN Fei-fei HUANG Hai-qing |
| author_facet | WANG Yi-zhen HAN Fei-fei HUANG Hai-qing |
| author_sort | WANG Yi-zhen |
| collection | DOAJ |
| description | Total RNA was extracted from mammary gland of mice. Two primers were designed and synthesized according to the murine lactoferrin gene reported in Genbank. A cDNA fragment about 897 bp in length was obtained through RT-PCR. The PCR product was cloned into pGEM-T vector and then sequenced. Sequence analysis suggested that this fragment corresponded to a portion of the sequence of lactoferrin cDNA and coded 299 amino acid residues, which constituted the major part of mature lactoferrin. The gene homology of fragment obtained in this study was 99% comparable to that of the reported lactoferrin cDNA sequence in uterus of mice. Based on the lactoferrin gene clone, we successfully constructed an optimal semi-quantitative RT-PCR method. Using β-actin as inner control, we detected the difference in expression of lactoferrin gene at different lactation stages of mice. The results showed that the amount of lactoferrin gene expression changed with lactation stages, it decreased from day 1 of lactation to day 18 of lactation, but during involution of mice mammary gland (day 25 of lactation), lactoferrin gene expression increased. |
| format | Article |
| id | doaj-art-877bc31dfe2c49dab068cc4091af62ac |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-877bc31dfe2c49dab068cc4091af62ac2025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-01-0130838810.3785/1008-9209.2004.01.008310089209Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stagesWANG Yi-zhenHAN Fei-feiHUANG Hai-qingTotal RNA was extracted from mammary gland of mice. Two primers were designed and synthesized according to the murine lactoferrin gene reported in Genbank. A cDNA fragment about 897 bp in length was obtained through RT-PCR. The PCR product was cloned into pGEM-T vector and then sequenced. Sequence analysis suggested that this fragment corresponded to a portion of the sequence of lactoferrin cDNA and coded 299 amino acid residues, which constituted the major part of mature lactoferrin. The gene homology of fragment obtained in this study was 99% comparable to that of the reported lactoferrin cDNA sequence in uterus of mice. Based on the lactoferrin gene clone, we successfully constructed an optimal semi-quantitative RT-PCR method. Using β-actin as inner control, we detected the difference in expression of lactoferrin gene at different lactation stages of mice. The results showed that the amount of lactoferrin gene expression changed with lactation stages, it decreased from day 1 of lactation to day 18 of lactation, but during involution of mice mammary gland (day 25 of lactation), lactoferrin gene expression increased.https://www.academax.com/doi/10.3785/1008-9209.2004.01.0083lactoferrinmicemammary glandgene clonesemi-quantitative RT-PCRgene expression |
| spellingShingle | WANG Yi-zhen HAN Fei-fei HUANG Hai-qing Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages 浙江大学学报. 农业与生命科学版 lactoferrin mice mammary gland gene clone semi-quantitative RT-PCR gene expression |
| title | Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| title_full | Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| title_fullStr | Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| title_full_unstemmed | Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| title_short | Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| title_sort | cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages |
| topic | lactoferrin mice mammary gland gene clone semi-quantitative RT-PCR gene expression |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0083 |
| work_keys_str_mv | AT wangyizhen cloningoflactoferringeneofmiceandthedifferenceoflactoferringeneexpressioninmammaryglandatdifferentlactationstages AT hanfeifei cloningoflactoferringeneofmiceandthedifferenceoflactoferringeneexpressioninmammaryglandatdifferentlactationstages AT huanghaiqing cloningoflactoferringeneofmiceandthedifferenceoflactoferringeneexpressioninmammaryglandatdifferentlactationstages |