Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3
Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monocl...
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MDPI AG
2024-08-01
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| Online Access: | https://www.mdpi.com/2073-4468/13/3/67 |
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| author | Wantanee Tommeurd Kanyarat Thueng-in Sirin Theerawatanasirikul Nongnaput Tuyapala Sukontip Poonsuk Nantawan Petcharat Nattarat Thangthamniyom Porntippa Lekcharoensuk |
| author_facet | Wantanee Tommeurd Kanyarat Thueng-in Sirin Theerawatanasirikul Nongnaput Tuyapala Sukontip Poonsuk Nantawan Petcharat Nattarat Thangthamniyom Porntippa Lekcharoensuk |
| author_sort | Wantanee Tommeurd |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monoclonal antibodies provide essential information for developing diagnostic assays and vaccine designs. In this study, monoclonal antibodies raised against FMD virus (FMDV) were produced. Sixty-six monoclonal antibodies demonstrated strong reactivity and specificity to FMDV. The purified monoclonal antibodies were further used for bio-panning to select phage expressing specific epitopes from phage-displayed 12 mer-peptide library. The phage peptide sequences were analyzed using multiple sequence alignment and evaluated by peptide ELISA. Two hybridoma clones secreted monoclonal antibodies recognizing linear epitopes on VP2 of FMDV serotype O. The non-neutralizing monoclonal antibody 6F4.D11.B6 recognized the residues 67–78 on antigenic site 2 resinding in VP2, while the neutralizing monoclonal antibody 8D6.B9.C3 recognized a novel linear epitope encompassing residues 115–126 on VP2. This information and the FMDV-specific monoclonal antibodies provide valuable sources for further study and application in diagnosis, therapeutics and vaccine designs to strengthen the disease prevention and control measures. |
| format | Article |
| id | doaj-art-8767bdccca0d45d9bf4462a7ba53bbf3 |
| institution | OA Journals |
| issn | 2073-4468 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj-art-8767bdccca0d45d9bf4462a7ba53bbf32025-08-20T01:55:57ZengMDPI AGAntibodies2073-44682024-08-011336710.3390/antib13030067Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3Wantanee Tommeurd0Kanyarat Thueng-in1Sirin Theerawatanasirikul2Nongnaput Tuyapala3Sukontip Poonsuk4Nantawan Petcharat5Nattarat Thangthamniyom6Porntippa Lekcharoensuk7Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, ThailandSchool of Pathology, Translational Medicine Program, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandInterdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, ThailandProtein-Ligand Engineering and Molecular Biology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, ThailandDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandResearch and Development Department, Animal Health and Diagnostic Center, CPF (Thailand) Public Company Limited, Bangkok 10530, ThailandInterdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, ThailandFoot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monoclonal antibodies provide essential information for developing diagnostic assays and vaccine designs. In this study, monoclonal antibodies raised against FMD virus (FMDV) were produced. Sixty-six monoclonal antibodies demonstrated strong reactivity and specificity to FMDV. The purified monoclonal antibodies were further used for bio-panning to select phage expressing specific epitopes from phage-displayed 12 mer-peptide library. The phage peptide sequences were analyzed using multiple sequence alignment and evaluated by peptide ELISA. Two hybridoma clones secreted monoclonal antibodies recognizing linear epitopes on VP2 of FMDV serotype O. The non-neutralizing monoclonal antibody 6F4.D11.B6 recognized the residues 67–78 on antigenic site 2 resinding in VP2, while the neutralizing monoclonal antibody 8D6.B9.C3 recognized a novel linear epitope encompassing residues 115–126 on VP2. This information and the FMDV-specific monoclonal antibodies provide valuable sources for further study and application in diagnosis, therapeutics and vaccine designs to strengthen the disease prevention and control measures.https://www.mdpi.com/2073-4468/13/3/67foot-and-mouth disease virusmonoclonal antibodyhybridomaphage display |
| spellingShingle | Wantanee Tommeurd Kanyarat Thueng-in Sirin Theerawatanasirikul Nongnaput Tuyapala Sukontip Poonsuk Nantawan Petcharat Nattarat Thangthamniyom Porntippa Lekcharoensuk Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 Antibodies foot-and-mouth disease virus monoclonal antibody hybridoma phage display |
| title | Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 |
| title_full | Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 |
| title_fullStr | Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 |
| title_full_unstemmed | Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 |
| title_short | Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3 |
| title_sort | identification of conserved linear epitopes on viral protein 2 of foot and mouth disease virus serotype o by monoclonal antibodies 6f4 d11 b6 and 8d6 b9 c3 |
| topic | foot-and-mouth disease virus monoclonal antibody hybridoma phage display |
| url | https://www.mdpi.com/2073-4468/13/3/67 |
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