The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany
<i>Proteus mirabilis</i> is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant <i>P. mirabil...
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2025-01-01
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| author | Moritz Fritzenwanker Jane Falgenhauer Torsten Hain Can Imirzalioglu Trinad Chakraborty Yancheng Yao |
| author_facet | Moritz Fritzenwanker Jane Falgenhauer Torsten Hain Can Imirzalioglu Trinad Chakraborty Yancheng Yao |
| author_sort | Moritz Fritzenwanker |
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| description | <i>Proteus mirabilis</i> is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant <i>P. mirabilis</i> isolates, including those producing ESBLs, AmpC cephalosporinases, and carbapenemases, are now more frequently reported. The most common carbapenemase types found in <i>P. mirabilis</i> are KPC-2, IMP, VIM, NDM, and OXA-48. We sequenced the genomes of three carbapenem-resistant <i>P. mirabilis</i> isolates harboring both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> from Germany using both short-read and long-read sequencing techniques. We found that the isolates were only distantly related genetically. Both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> genes were located on a class I integron, which in two cases was located on the chromosome and in one case on a plasmid. This is the first report on the complete genomes of <i>P. mirabilis</i> strains harboring a rare genetic element encoding both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub>. Our results emphasize a key role for class 1 integrons in the transmission of VIM carbapenemases in <i>P. mirabilis</i>. |
| format | Article |
| id | doaj-art-87534f90c93e457fb894ca7925d8ea19 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-87534f90c93e457fb894ca7925d8ea192025-08-20T03:12:19ZengMDPI AGMicroorganisms2076-26072025-01-0113226610.3390/microorganisms13020266The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in GermanyMoritz Fritzenwanker0Jane Falgenhauer1Torsten Hain2Can Imirzalioglu3Trinad Chakraborty4Yancheng Yao5Institute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, Germany<i>Proteus mirabilis</i> is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant <i>P. mirabilis</i> isolates, including those producing ESBLs, AmpC cephalosporinases, and carbapenemases, are now more frequently reported. The most common carbapenemase types found in <i>P. mirabilis</i> are KPC-2, IMP, VIM, NDM, and OXA-48. We sequenced the genomes of three carbapenem-resistant <i>P. mirabilis</i> isolates harboring both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> from Germany using both short-read and long-read sequencing techniques. We found that the isolates were only distantly related genetically. Both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> genes were located on a class I integron, which in two cases was located on the chromosome and in one case on a plasmid. This is the first report on the complete genomes of <i>P. mirabilis</i> strains harboring a rare genetic element encoding both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub>. Our results emphasize a key role for class 1 integrons in the transmission of VIM carbapenemases in <i>P. mirabilis</i>.https://www.mdpi.com/2076-2607/13/2/266<i>Proteus mirabilis</i>carbapenemasesVIM-75 and VIM-4multi-drug-resistanceplasmid |
| spellingShingle | Moritz Fritzenwanker Jane Falgenhauer Torsten Hain Can Imirzalioglu Trinad Chakraborty Yancheng Yao The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany Microorganisms <i>Proteus mirabilis</i> carbapenemases VIM-75 and VIM-4 multi-drug-resistance plasmid |
| title | The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany |
| title_full | The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany |
| title_fullStr | The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany |
| title_full_unstemmed | The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany |
| title_short | The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany |
| title_sort | detection of extensively drug resistant i proteus mirabilis i strains harboring both vim 4 and vim 75 metallo β lactamases from patients in germany |
| topic | <i>Proteus mirabilis</i> carbapenemases VIM-75 and VIM-4 multi-drug-resistance plasmid |
| url | https://www.mdpi.com/2076-2607/13/2/266 |
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