The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany

The Detection of Extensively Drug-Resistant <i>Proteus mirabilis</i> Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany

<i>Proteus mirabilis</i> is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant <i>P. mirabil...

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Main Authors: Moritz Fritzenwanker, Jane Falgenhauer, Torsten Hain, Can Imirzalioglu, Trinad Chakraborty, Yancheng Yao
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microorganisms
Subjects:
<i>Proteus mirabilis</i>
carbapenemases
VIM-75 and VIM-4
multi-drug-resistance
plasmid
Online Access:https://www.mdpi.com/2076-2607/13/2/266
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Summary:<i>Proteus mirabilis</i> is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant <i>P. mirabilis</i> isolates, including those producing ESBLs, AmpC cephalosporinases, and carbapenemases, are now more frequently reported. The most common carbapenemase types found in <i>P. mirabilis</i> are KPC-2, IMP, VIM, NDM, and OXA-48. We sequenced the genomes of three carbapenem-resistant <i>P. mirabilis</i> isolates harboring both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> from Germany using both short-read and long-read sequencing techniques. We found that the isolates were only distantly related genetically. Both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub> genes were located on a class I integron, which in two cases was located on the chromosome and in one case on a plasmid. This is the first report on the complete genomes of <i>P. mirabilis</i> strains harboring a rare genetic element encoding both <i>bla</i><sub>VIM-4</sub> and <i>bla</i><sub>VIM-75</sub>. Our results emphasize a key role for class 1 integrons in the transmission of VIM carbapenemases in <i>P. mirabilis</i>.
ISSN:2076-2607

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