Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue
Background and aims: Gastric cancer is a major public health issue as the fourth leading cause of cancer-related death in the world. Reactive oxygen species (ROS) induce DNA damage and this process plays an important role in gastric cancer development and progression. OGG1 is an essential component...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Iran Air Force Health Administration
2021-07-01
|
| Series: | فصلنامه ابن سینا |
| Subjects: | |
| Online Access: | http://ebnesina.ajaums.ac.ir/article-1-942-en.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850072415977078784 |
|---|---|
| author | Somayyeh Moosaie Zeynab Afshari Abdollah Abbasi Manuchehr Ghojaie Arezoo Farhadi Mohammad Foad Heidari Shirin Shahbazi Javad Behroozi Mehrdad Nasrollahzadeh Sabet |
| author_facet | Somayyeh Moosaie Zeynab Afshari Abdollah Abbasi Manuchehr Ghojaie Arezoo Farhadi Mohammad Foad Heidari Shirin Shahbazi Javad Behroozi Mehrdad Nasrollahzadeh Sabet |
| author_sort | Somayyeh Moosaie |
| collection | DOAJ |
| description | Background and aims: Gastric cancer is a major public health issue as the fourth leading cause of cancer-related death in the world. Reactive oxygen species (ROS) induce DNA damage and this process plays an important role in gastric cancer development and progression. OGG1 is an essential component of the base excision repair pathway that is required for the removal of oxidized guanine nucleotides from DNA exposed to ROS. This study aimed to compare the expression of the OGG1 gene in cancerous and healthy tissues of gastric cancer.
Methods: Fifty pairs of tumors and adjacent normal tissues were collected from gastric cancer patients. Total RNA was extracted and complementary DNA (cDNA) was synthesized. The relative gene expression of OGG1 was determined using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). A data mining study was also performed to determine the prognostic role of OGG1 expression in the overall survival of gastric cancer patients.
Results: OGG1 gene expression was significantly increased in patients' tumor samples compared to normal adjacent tissue samples. In addition, the expression of OGG1 in patients with early stages of gastric cancer was significantly higher than those with advanced stages. Also, a negative correlation was observed between the high expression of OGG1 and the overall survival rate.
Conclusion: It can be concluded that the expression of OGG1 increases in the early stages of gastric cancer, which could be related to the increase in oxidative damage to DNA. |
| format | Article |
| id | doaj-art-8746aaea1fdf430a862a902f97053b67 |
| institution | DOAJ |
| issn | 1735-9503 2645-4653 |
| language | fas |
| publishDate | 2021-07-01 |
| publisher | Iran Air Force Health Administration |
| record_format | Article |
| series | فصلنامه ابن سینا |
| spelling | doaj-art-8746aaea1fdf430a862a902f97053b672025-08-20T02:47:05ZfasIran Air Force Health Administrationفصلنامه ابن سینا1735-95032645-46532021-07-012325866Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissueSomayyeh Moosaie0Zeynab Afshari1Abdollah Abbasi2Manuchehr Ghojaie3Arezoo Farhadi4Mohammad Foad Heidari5Shirin Shahbazi6Javad Behroozi7Mehrdad Nasrollahzadeh Sabet8 Department of Biology, Faculty of Science, Bonab Branch, Islamic Azad University, Bonab, Iran Department of Biology, Faculty of Science, Bonab Branch, Islamic Azad University, Bonab, Iran Department of Biology, Faculty of Science, Bonab Branch, Islamic Azad University, Bonab, Iran Department of Biology, Faculty of Science, Bonab Branch, Islamic Azad University, Bonab, Iran Department of Genetics and Biotechnology, Faculty of Life Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran Department of Medical Laboratory Sciences, School of Allied Health Medicine, Aja University of Medical Sciences, Tehran, Iran Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Genetics and Biotechnology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran Department of Genetics and Biotechnology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran Background and aims: Gastric cancer is a major public health issue as the fourth leading cause of cancer-related death in the world. Reactive oxygen species (ROS) induce DNA damage and this process plays an important role in gastric cancer development and progression. OGG1 is an essential component of the base excision repair pathway that is required for the removal of oxidized guanine nucleotides from DNA exposed to ROS. This study aimed to compare the expression of the OGG1 gene in cancerous and healthy tissues of gastric cancer. Methods: Fifty pairs of tumors and adjacent normal tissues were collected from gastric cancer patients. Total RNA was extracted and complementary DNA (cDNA) was synthesized. The relative gene expression of OGG1 was determined using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). A data mining study was also performed to determine the prognostic role of OGG1 expression in the overall survival of gastric cancer patients. Results: OGG1 gene expression was significantly increased in patients' tumor samples compared to normal adjacent tissue samples. In addition, the expression of OGG1 in patients with early stages of gastric cancer was significantly higher than those with advanced stages. Also, a negative correlation was observed between the high expression of OGG1 and the overall survival rate. Conclusion: It can be concluded that the expression of OGG1 increases in the early stages of gastric cancer, which could be related to the increase in oxidative damage to DNA.http://ebnesina.ajaums.ac.ir/article-1-942-en.pdfgastric cancerhogg1 enzymegene expressionreal-time pcr |
| spellingShingle | Somayyeh Moosaie Zeynab Afshari Abdollah Abbasi Manuchehr Ghojaie Arezoo Farhadi Mohammad Foad Heidari Shirin Shahbazi Javad Behroozi Mehrdad Nasrollahzadeh Sabet Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue فصلنامه ابن سینا gastric cancer hogg1 enzyme gene expression real-time pcr |
| title | Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| title_full | Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| title_fullStr | Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| title_full_unstemmed | Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| title_short | Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| title_sort | comparison of ogg1 gene expression level in gastric adenocarcinoma and adjacent normal tissue |
| topic | gastric cancer hogg1 enzyme gene expression real-time pcr |
| url | http://ebnesina.ajaums.ac.ir/article-1-942-en.pdf |
| work_keys_str_mv | AT somayyehmoosaie comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT zeynabafshari comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT abdollahabbasi comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT manuchehrghojaie comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT arezoofarhadi comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT mohammadfoadheidari comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT shirinshahbazi comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT javadbehroozi comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue AT mehrdadnasrollahzadehsabet comparisonofogg1geneexpressionlevelingastricadenocarcinomaandadjacentnormaltissue |